User manual BIOPTECHS FCS2PERFUSION

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Manual abstract: user guide BIOPTECHS FCS2PERFUSION

Detailed instructions for use are in the User's Guide.

 [. . . ]  [. . . ] Typical FCS2® Perfusion Configuration for Induced Change Experiments

1 Perfusion tubing,  1/16" ID supply line (keep as short as possible) 2 FCS2 Micro-Perfusion Pump (recommended for prolonged,  controlled perfusion in delicate optical cavities) 3 Tee adapter (adds low volume syringe to facilitate manual perfusion when loading chamber) 4 Luer adapter (female,  adapts Luer taper syringe to 1/16" tubing) 5 Low volume glass syringe

6 7 8 9 10 11

Luer adapter (male,  adapts 1/16" tubing to female Luer lock) Alternate media glass syringe (growth factors,  inhibitors,  etc. ) Three-way valve (switches source of perfusion; pump or manual) FCS2 Chamber Tubing to waste container Tee adapter (place at same height as chamber,  open top of tee to prevent siphoning)

3560 Beck Road · Butler,  PA 16002 · Voice: 724-282-7145 · Fax: 724-282-0745 · E-mail: info@bioptechs. com · Web: www. bioptechs. com

Note: Syringes are glass to prevent contamination of media from silicone lubricant typical in plastic syringes.  [. . . ]  [. . . ]

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