User manual FLUORESCENCE VC-CATALOG

[. . . ] Vacu-Cell TM Incubation Chamber System* C&L introduces the Vacu-Cell line of cell incubation chambers. This unique chamber design holds a coverslip against the chamber body by one side only, providing major advantages over other types of cell chambers. Several models are available to fit your specific application. Advantages of the Vacu-Cell: - Complete access to the bottom of the chamber, all the way to the edges. [. . . ] A port for a temperature sensor is provided. Temperature is controlled using a heated microscope stage adapter. Top and bottom vacuum ports 48 x 65 mm coverslip (bottom view, without bottom coverslip) Model VC-RPC-LW Model VC-RPC-LW (top view) Model Numbers Model VC-RPC-LW (sectioned view) Large Window RPC, Black Anodized Aluminum VC-RPC-LW-BA Rectangular 48 x 65 mm No. 1 Coverslips, 1 Box VC-48x65CS-1 VC-RPC-LW-ORINGS Replacement O-rings for RPC-LW, 5 Sets C&L Instruments, Inc. Patent Pending www. fluorescence. com 717-564-9491 Vacu-CellTM System Rectangular Perfusion Chamber, Thin Window Model RPC-TW This Perfusion Chamber uses two rectangular coverslips to form a thin perfusion chamber. Two sets of dual o-rings on the bottom of the chamber body securely hold a 24 x 40 mm (upper) and a 48 x 65 mm (lower) coverslip in place. Each coverslip is held in place using a separate vacuum port, so they can be used independently. The observation window measures 0. 44 x 1. 04 inches (11. 2 x 26. 4 mm). When the Model RPC-TW is used with two coverslips, a rectangular laminar flow chamber is formed with the two coverslips separated 100 microns, creating a thin laminar flow chamber. Inlet and outlet perfusion ports direct fluid flow from one end to the other between the two coverslips in a laminar fashion. Either the upper or lower coverslip can be used without the other to fashion an open chamber configuration. This chamber is available in black anodized aluminum that is coated with parylene for biological compatibility. A port for a temperature sensor is provided. Temperature is controlled using a heated microscope adapter. Top and bottom vacuum ports The observation window measures 0. 44 x 1. 04 inches (bottom view, without coverslips attached) Model VC-RPC-TW Model VC-RPC-TW (top view) Model Numbers VC-RPC-TW-BA VC-48x65CS-1 VC-24x40CS-1 VC-RPC-TW-ORINGS Thin Window RPC, Black Anodized Aluminum Rectangular 48 x 65 mm No. 1Coverslips, 1 Box Rectangular 24 x 40 mm No. 1Coverslips, 1 Box O-rings for RPC-TW, 5 Sets Model VC-RPC-TW (sectioned view) C&L Instruments, Inc. www. fluorescence. com 717-564-9491 Patent Pending Vacu-CellTM System Mini Perfusion Chamber, Open Top Model MPC-OT The Mini Series Open Top Chamber uses a single 25 mm round coverslip. Dual o-rings on the bottom of the chamber securely hold the coverslip in place using a vacuum seal. The observation window measures 17. 5 mm diameter. The total volume in the observation window is approximately 240 µl when the depth of the perfusion fluid is 1 mm. The perfusion ports are configured for use with 1/16 inch OD tubing. This chamber is available in black anodized aluminum that is coated with parylene for biological compatibility. Temperature is controlled using a heated microscope stage adapter. Vacuum port The observation window measures 17. 5 mm diameter Model VC-MPC-OT (sectioned view) Perfusion port Model VC-MPC-OT (top view) Model Numbers VC-MPC-OT-BA VC-25RCS-1 VC-25RCS-2 VC-MPC-OT-ORINGS Large Window MPC, Black Anodized Aluminum 25 mm No. [. . . ] Heated stage holders made of aluminum are available for the Rectangular and Mini Vacu-Cell series chambers. Model VC-LFR-18-H Vacu-Cell shown in a Model VC-RSH-NIK Nikon microscope stage holder Model VC-OTR-25 Vacu-Cell shown in a Model VC-RSH-NIK Nikon microscope stage holder Model VC-CPR-25 Vacu-Cell shown in a Model VC-RSH-NIK Nikon microscope stage holder Model Numbers VC-RSH-OLY Stage holder for Olympus microscope VC-RSH-NIK Stage holder for Nikon microscope VC-RSH-ZEI Stage holder for Zeiss microscope (Specify Model Number when ordering) C&L Instruments, Inc. Patent Pending www. fluorescence. com 717-564-9491 Vacu-CellTM System 24 Well Microtitre Vacu-CellTM Chamber Model VC-MT24 The Model MT24 microtitre chamber uses one 48 x 60 mm coverslip to form 24 individual incubation chambers. Multiple o-rings on the bottom of the chamber body securely hold the coverslip in place using a vacuum. The size of the individual chambers is 2. 6 mm in diameter and the cylindrical portion of each chamber holds approximately 25 µl. The chambers are spaced on a 9 x 9 mm grid. [. . . ]