User manual HANNA INSTRUMENTS HI 4004

[. . . ] HI 4104 CALCIUM COMBINATION HI 4000-50 SENSOR HANDLE Instruction Manual HI 4004 HI 4104 Calcium Ion Selective Electrode Half-cell Combination 1 HI 4004 Calcium Half-cell HI 4104 Calcium Combination Electrode I. Introduction: The Hanna HI 4004 and HI 4104 are ion selective electrodes designed for the measurement of calcium ions in aqueous solutions. They utilize a replaceable sensing module that contains an organic polymer membrane that is sensitive to calcium ions. The HI 4004 is a half-cell electrode that requires a separate reference. [. . . ] Soak the Calcium electrodes membrane in HI 4004-45 conditioning solution or a calcium standard (0. 01M) without ISA before calibration. HI 4104 The Hanna HI 4104 is shipped disassembled. The sensing module is found in the glass storage vial. Unwrap Parafilm® seal found over ceramic junction on inner stem and discard. This is only used for shipping or long term storage. Do not touch the sensing membrane with the "H" hole pattern on it. Screw the cone into the inner stem finger tight. Do not over tighten. 8 Deionized Water Remove Parafilm Install sensing module 4. Rinse inner stem with deionized water making certain to wet o-ring found on the inner stem. Reassemble electrode by gently pushing the inner assembly into the outer body, sliding spring down cable, and screwing cap into place. Remove fill hole cover and o-ring on fill hole spout. Using the dropper pipette provided, add a few drops HI 7082 fill solution to the electrode. Invert the electrode to wet the o-ring and rinse the fill solution chamber. 9 7. Holding the body of the electrode gently press upper cap with your thumb. This permits the fill solution to drain out of the body. Release cap and verify electrode returns to its original position. (You may need to gently assist for this to occur). 8. Tighten the electrode cap onto the body and fill electrode body until fill solution volume is just below fill hole. Position electrode in a Hanna HI 76404 electrode holder (or equivalent) and connect BNC connector to meter. · · · Quick Check of Electrode Slope Connect electrode(s) to pH/mV/ISE meter Place meter in mV mode. Place 100 mL of deionized water into a beaker with stir bar. · Place reference and measuring half-cell or combination electrode into prepared sample. [. . . ] Standards should bracket and fall within the range of interest. Standards and solutions should be at the same temperature. 2) 2 mL of HI 4004-00 is added to 100 mL of both samples and standards. Add stir bar and mix before taking measurements. [. . . ]