User manual LEICA CM1950

[. . . ] Cryostat Operating Manual Leica CM1950 , Rev C, English, 1v3 ­ 04/2010 Always keep this manual near the instrument. Read carefully prior to working with the instrument! 1. NOTE The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. [. . . ] Only then can the UV lamp be switched back on. * Leica Cryofect is not available in all countries. Leica CM1950 ­ Cryostat 35 8. 8. 1 Working with the Instrument Preparing cutting tools, specimen discs and preparation aids The knives are extremely sharp!Never try to catch a falling knife! · Place working materials such as the blade box or knives (in the knife case), brush, pincers or preparation needles and, where applicable, specimen discs into the cryostat chamber. · The necessary tools and preparation aids can be precooled on the (optional) movable shelf, making them available at all times in a convenient position for the user. · In addition, specimen discs can be precooled and stored in the storage system (see page 26, Fig. 21). For installation of knife/blade holder and installation in the chamber, refer to optional accessories on page 53) Fig. 36 8. 2 Turning the instrument on The instrument must be switched on at least 5 hours before the planned use. The circuit breaker is used as power switch. The switch must be in the top position for switching on and in the bottom position for switching off. The switch must be accessible without obstruction. · Close the sliding window. Fig. 37 To avoid frost formation always put the cover on the quick freeze shelf. Always cover the quick freeze shelf during breaks and overnight. 36 Operating Manual, Rev C, 1v3 ­ 04/2010 8. 8. 3 Configuring the parameters Working with the Instrument The instrument must be switched on at least 5 hours before the planned use. · Turn the lamp on or off. · This button activates or deactivates the manual defrost of the cooling chamber, specimen head or freeze shelf. (For more detailed handling instructions, refer to the chapter on "Working with the Instrument" on p. 39 of this Operating Manual. ) · Pressing the key button for approx. locks the entire keyboard (the LEDs in the clock go out). · Pressing the key button briefly, then the "-" key in the specimen head control panel field switches off the specimen head. · Pressing the key button briefly, then the "+" key in the specimen head control panel field switches the specimen head back on. Programming the temperature of the cryochamber · The temperature of the cryochamber is set and indicated on the control panel marked with the cryostat symbol. The actual temperature is the standard indication. Briefly pressing the "+" or "-" button displays the target temperature. Set the desired value via the "+" / "-" buttons. Pushing the "+" or "-" button for more than 1 sec. increases or decreases the chamber temperature continuously. [. . . ] · Hold the lamp with both hands and pull it to the right out of the holder (4). Fig. 74 3 4 Only use lamps of the same specification! Installation of the new lamp Fig. 75 Type: OSRAM DULUX L 18W/ 840. · Hold the lamp in the correct mounting position as shown and push it to the right until it engages in the holder. 72 Operating Manual, Rev C, 1v3 ­ 04/2010 13. Decontamination Certificate (master) Dear Customer, Any product which is to be returned to Leica Biosystems or serviced on site, must be cleaned and decontaminated in the appropriate manner. [. . . ]