User manual LEICA CN

[. . . ] This document is protected under copyright laws. Any copyrights of this document are retained by Leica Microsystems Nussloch GmbH. Any reproduction of text and illustrations (or of any parts thereof) by means of print, photocopy, microfiche, web cam or other methods ­ including any electronic systems and media ­ requires express prior permission in writing by Leica Microsystems Nussloch GmbH. For the instrument serial number and year of manufacture, please refer to the name plate at the back of the instrument. © Leica Microsystems Nussloch GmbH Published by: Leica Microsystems Nussloch GmbH Heidelberger Str. [. . . ] The knife height is adjusted via the knurled nuts (16). The upper edge of the back plate (rear clamping plate) serves as an index to determine the appropriate knife height. The knife edge should be at the same level as the back plate; even knives that have been greatly reduced in height by frequent resharpening (up to a height of 25 mm) can be properly height-adjusted and clamped in the knife holder CN. 15 16 16 Fig. 7 18 17 The anti-roll system is laterally adjustable (84-mm wide system only). A groove (17) in the axle (18) facilitates centering of the anti-roll system. Fig. 8 6 Instruction manual V 2. 2 ­ 08/2005 1. 19 Operation Finger guards The finger guards are integrated into the clamping jaws. Use handles (19) to slide them back and forth. The width of the finger guards is sufficient for knives up to 22 cm long. Once you finish sectioning, always cover the knife edge with the finger guards. Fig. 9 Prior to any work on knife and object, each time before changing the specimen block and during work breaks the knife edge must be covered with the finger guards! Shifting the clamping jaws 20 21 21 In the factory, the clamping jaws are mounted at a distance of 64 mm from each other. If necessary, the clamping jaws can be relocated to a distance of 84 mm. Fig. 10 23 22 To shift the clamping jaws the knife holder must be removed from the knife holder base. Loosen lever (20) and remove knife holder from base. With a size 4 Allen key, loosen the screws (21) located at the bottom surface of the knife holder. Shift the clamping jaws (22) and retighten the screws. Insert the longer of the two knife support bars (part of standard delivery). Never work with just one clamping jaw. Otherwise sufficient stability for the sectioning process cannot be guaranteed. In addition, long knives will no longer be sufficiently covered by the finger guard. Fig. 11 Leica knife holder CE /CN 7 1. Operation Knife support bar Ensure that the recess (24) is pointing away from the microtome. Microtome knives and blades have extremely sharp cutting edges!Never try to catch a falling knife! 24 Fig. 12 Clearance angle adjustment The harder the specimen the wider the clearance angle. However: the wider the clearance angle the greater the compression of the sections. [. . . ] Loosen clamping lever (35) of pressure plate. Now you can remove the pressure plate (36) for cleaning (use alcohol or acetone). 34 36 35 For disinfection purposes, standard commercial detergents and disinfectants can be used ­ our recommendation is Leica Cryofect. Fig. 19 38 39 Cleaning the knife holder CN Daily cleaning procedure For daily cleaning, it is sufficient to remove section waste from the knife holder with a dry brush. Make sure to use a cold brush, otherwise the sections will thaw and stick to the knife holder. 37 Thorough cleaning From time to time, all movable parts should be removed for cleaning and subsequent lubrication with cryostat oil, type 407. Fig. [. . . ]