User manual LEICA CRYOFECT

[. . . ] The system's individual components can be used in a variety of embedding procedures to perfectly embed and properly orient almost any type of specimen. The process is comfortably performed inside the cryostat using stainless steel well bars, chucks, and freezing blocks. Since the Precision Cryoembedding System's components are stored at cold temperatures, most specimens freeze in 20 to 60 seconds, depending upon their size and the selected freezing technique. [. . . ] This significantly reduces turn-around time. And, your well-oriented, flat specimens will be conserved during the trimming process!What else could you ask for? Cryoembedding components in the Leica CM1850 cryochamber The Precision Cryoembedding System consists of: · 3 well bars in 3 sizes (18, 24 and 30 mm) · 6 small stages (chucks) · 4 large stages (chucks) · 4 over-stage heat extractors · 1 storage bin for stages · 16 dispensing slides · 1 cutting board/freezing griddle · 1 elevated heat extractor · 1 pair of angled embedding forceps and accessories Precision cryoembedding accessories Face Down Cryoembedding Perfect, Flat Orientation Any specimen, whether it is single or multiple pieces; large or minute; solid or liquid, can be embedded flat and in a single plane. Technique 1. Precisely orient the specimen(s), face down, on a thin plastic dispensing slide. Touch the edge of a specimen to the cold base of the embedding well and gently withdraw the dispensing slide while positioning the specimen. Slightly overfill the well with embedding medium. Place an over-chuck freezing block over the stem of the chuck. Allow an end of the paper to overhang the edge of the dispensing slide. Touch the lens paper to the cold floor of the embedding well and gently pull the dispensing slide away. The specimen will remain correctly positioned on the lens paper. Trim through the paper on the trimming portion of the blade and then move to a clean, sharp portion to section the specimen. (Untrimmed block in upper portion of photo #4, trimmed block in lower portion. ) 1 2 3 4 The Results A: Large undissected specimen, dispensing slide and lens paper B: Specimen dissected with inked border C: Embedded block D: Stained sections on slide A B C D Leica Microsystems ­ the brand for outstanding products Leica Microsystems' mission is to be the world's first-choice provider of innovative solutions to our customers' needs for vision, measurement, lithography and analysis of microstructures. Leica, the leading brand for microscopes and scientific instruments, developed from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and Cambridge Instruments. Yet Leica symbolizes innovation as well as tradition. The companies of the Leica Microsystems Group operate internationally in four business segments, where we rank with the market leaders. · Microscopy Systems Leica Microsystems ­ an international company with a strong network of customer services Australia: Austria: Canada: Denmark: France: Germany: Italy: Japan: Korea: Netherlands: People's Rep. [. . . ] Yet Leica symbolizes innovation as well as tradition. The companies of the Leica Microsystems Group operate internationally in four business segments, where we rank with the market leaders. · Microscopy Systems Leica Microsystems ­ an international company with a strong network of customer services Australia: Austria: Canada: Denmark: France: Germany: Italy: Japan: Korea: Netherlands: People's Rep. of China: Portugal: Singapore Spain: Sweden: Switzerland: United Kingdom: USA: Gladesville Vienna Richmond Hill/Ontario Herlev Rueil-Malmaison Bensheim Milan Tokyo Seoul Rijswijk Hong Kong Lisbon Barcelona Sollentuna Glattbrugg Milton Keynes Bannockburn/lllinois Tel. +1 847 405 0123 Fax +61 2 9817 8358 Fax +43 1 486 80 50 30 Fax +1 905 762 8937 Fax +45 4454 0111 Fax +33 1 473 285 86 Fax +49 6251 136 155 Fax +39 0257 40 3273 Fax +81 3 5435 9615 Fax +82 2 514 65 48 Fax +31 70 4132 109 Fax +852 2564 4163 Fax +351 21 385 4668 Fax +65 6773 0628 Fax +34 93 494 95 32 Fax +46 8 625 45 10 Fax +41 1 809 34 44 Fax +44 1908 609 992 Fax +1 847 405 0164 Our expertise in microscopy is the basis for all our solutions for visualization, measurement and analysis of microstructures in life sciences and industry. [. . . ]