User manual LEICA MEASURING

[. . . ] can be taken with the aid of the graticule with co-ordinate grid and the angular division on the rotary polarisation table. Please refer to the instrument's directions for use with regard to using the graticule in eyepieces. Two adjustable eyepieces are required for equipment with measuring graticules so that the sharpness can be set precisely. If you take digital photographs with your stereomicroscope or macroscope, we would recommend the Leica IM image archiving and image management software with measuring module for interactive measuring, inscribing and marking. Counting Calibrating Angle measurements Eyepieces Software for measuring tasks 2 Measuring ­ User Manual 0 1 2 3 4 5 6 7 8 9 10 1 1 12 12 mm : 120 0. 01 mm 2 0. 1 mm 0. 5 mm 0 1 mm 3 1 0 20 40 60 80 100 100 x 1 mm2 2 5 mm : 100 50 mm 1 Care, cleaning Avoid corrosive cleaning agents and materials which will scratch the surfaces. [. . . ] Observe specimen through both eyepieces. If necessary, use the focusing drive to re-focus. Checking eyepiece is free of parallax Look into the eyepieces and slightly move your head. · Graticule and specimen must not move towards one another, i. e. they must be clearly focused in one plane (free of parallax). If this is not the case, please repeat the process. Measuring ­ User Manual 5 Calibrating graticules Why calibrate?The graticule is only magnified by the eyepiece. The total magnification of the specimen does however depend on the lens, magnification changer, eyepiece and additional tube possibly used (e. g. coaxial lighting sources) and changes whenever these factors are altered. When calibrating with the stage micrometer, the value of an interval on the graticule is defined in relation to the specimen magnification. The true dimensions of a specimen can only be established once the calibrated value has been defined. You do not have to calibrate You have to calibrate Only calibrate once . . . if you only undertake relative measurements or comparisons. if you need absolute measurement values. The calibration value of each of your optics/ magnification combinations used only has to be established once. Keep a table of the appropriate calibration values (refer to example on page 12). When working with instruments with engageable zoom settings (Leica MZ6, MZ75, MZ95 etc. ) or adjustable stops (Leica S6 models etc. ), the specific magnifications can always be re-produced exactly (refer to directions for usage for the stereomicroscope). When using instruments with zoom magnification changers without engageable setting stages, inaccuracies could arise when again undertaking settings. If a high degree of measurement precision is required, you should therefore undertake calibration whenever changing magnification. Engaging zoom settings 6 Measuring ­ User Manual Calibrating Place stage micrometer on the table insert and set sharpness. Select the magnification with which you will later want to take measurements. [. . . ] Mesh pitch 100 × 1mm2 y 9 20 1 2 3 4 5 6 7 x Calibrating 6. 2mm on the stage micrometer (X) 10 intervals on the eyepiece graticule (Y) 6. 2 mm = 0. 62mm calibration value 10 A B Counting Area of boxed in square A: 0. 38mm2 Result: 4 cells per 0. 38mm2 Area of boxed in square B: 9. 5mm2 Result: 16 cells per 9. 5mm 2 Measuring ­ User Manual 11 Table Calibrating 1. Count: number of mm (inches) on the stage micrometer (X) number of intervals on the eyepiece graticule (Y) 3. Calculate calibration value (one interval of the eyepiece graticule): X Y = mm (inch) calibration value Illustrations, descriptions and technical data are not binding and may be changed without notice. M2-275-0en · © Leica Microsystems (Switzerland) Ltd · CH-9435 Heerbrugg, 2001 · Printed in Switzerland ­ V. 2004 ­ RDV Measuring 4. [. . . ]