User manual LEICA MM MULTI WAVELENGTH CELL SCORING DATASHEET

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Manual abstract: user guide LEICA MM MULTI WAVELENGTH CELL SCORINGDATASHEET

Detailed instructions for use are in the User's Guide.

[. . . ] Leica MM Multi Wavelength Cell Scoring powered by MetaMorph® Analysis Software Drop-in for Leica MM AF · Multi-Parameter analysis of up to seven wavelengths · Identification of subpopulations of cells · Adaptive background correctionTM for improved segmentation · Field and Cell-by-Cell measurements The Leica MM Multi Wavelength Cell Scoring Application Module for Leica MM AF software is designed for the scoring of cells with up to seven fluorescent stains. [. . . ] The simple interface minimizes setup efforts and at the same time enables users to customize the settings and measurements to obtain the best possible results specific to the type of cells used in the experiments. Multiple wavelengths Leica MM AF provides a flexible integrated solution for multi wavelength image acquisition and analysis. Up to seven wavelengths can be analyzed simultaneously by the module. U2OS rat -arrestin 2-RrGFP cells treated with 1 m isoproterenol. Blue: Hoechst 33342, red: AntiPhospho-Histone H3 (Ser28), green: Transfluor® vesicles. Configuration for analysis Set the number of wavelengths used and repeat the following steps for each wavelength: 1. Choose the stained area (nucleus, cytoplasm, or both) 3. Specify approximate minimum and maximum widths of the objects to be detected 4. Adjust detection sensitivity by specifying the intensity above local background 5. Set positive scoring criterion (minimum stained area) 6. [. . . ] All settings can be saved for future experiments. www. leica-microsystems. com [. . . ]

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