User manual LEICA PRECISION CRYOEMBEDDING SYSTEM BROCHURE

[. . . ] Precision Cryoembedding System Frozen Sections Become a Work of Art! Precision Cryoembedding System Easily achieve proper specimen orientation and perfect embedding with the unique Precision Cryoembedding System. The Precision System was developed by American pathologist, Dr. Stephen Peters, to expedite and improve frozen sections and shorten the learning process for pathology residents. [. . . ] Place an over-chuck freezing block over the stem of the chuck. Remove freezing block and tap the chuck stem when freezing is complete (usually 20 to 60 seconds) to remove the embedded block from the embedding well. 1 2 3 4 5 6 The Results A: Trimmed block B: Stained section on slide C: Photomicrograph A B C Frozen Block Cryoembedding Precise, On-edge Orientation Specimens are embedded and frozen in their entirety, then mapped and cut into firm, flat pieces. The still frozen, flat pieces are then embedded on edge. This technique is perfect for flimsy, tubular, curled, or angular specimens and it is particularly useful for margin resections. 1 Technique 1. Place the specimen face down on the freezing griddle. Cover the specimen with a layer of embedding medium. Cover the specimen with the appropriate elevated freezing block. When completely frozen, map the specimen. Cut the embedded block into pieces on the cold cutting board, keeping pieces cold on the adjacent metal surface. (The main photo, #5 to the left, shows the central section while the inset shows a longitudinal margin. ) 6. Place the cut pieces face down (pieces are face up in the inset photo #6) in the embedding well and freeze using the procedure described under Face Down Cryoembedding. 2 3 4 5 6 The Results A: Trimmed block B: Stained section on slide C: Photomicrograph (sections repositioned in photograph) A B C Paper Cryoembedding Proper Positioning of Difficult Specimens This technique is used to maintain the orientation of delicate or flimsy specimens or to arrange multiple specimens so they will remain in the same plane for sectioning. Technique 1. Soak a small piece of lens paper in embedding medium and flatten it to the dispensing slide. Place the specimen(s) on the lens paper and orient appropriately. Allow an end of the paper to overhang the edge of the dispensing slide. [. . . ] With confocal laser technology and image analysis systems, we provide threedimensional viewing facilities and offer new solutions for cytogenetics, pathology and materials sciences. · Specimen Preparation · Medical Equipment Innovative technologies in our surgical microscopes offer new therapeutic approaches in microsurgery. @ www. histo-solutions. com Leica Microsystems Nussloch GmbH Heidelberger Strasse 17-19 D-69226 Nussloch Telephone: (06224) 143-0 Fax: (06224) 143 200 e-mail: histo_info@leica-microsystems. com www. leica-microsystems. com 09/2004 and representatives of Leica Microsystems in more than 100 countries. · Semiconductor Equipment Our automated, leading-edge measurement and inspection systems and our E-beam lithography systems make us the first choice supplier for semiconductor manufacturers all over the world. ©Leica Microsystems Nussloch GmbH, Heidelberger Str. (06224) 143-0, Fax (06224) 143-200 We provide comprehensive systems and services for clinical histo- and cytopathology applications, biomedical research and industrial quality assurance. Our product range includes instruments, systems and consumables for tissue infiltration and embedding, microtomes and cryostats as well as automated stainers and coverslippers. Order No. [. . . ]