User manual LEICA TCS LSI BROCHURE

[. . . ] Leica TCS LSI The World`s First Super Zoom Confocal!From Gene to Cell - from Cell to Embryo 1 2 · · · · The World`s First Super Zoom Confocal In Vivo - Large Scale Imaging Freedom for New Reserach Easy to Achieve 2 An increasing number of scientists extend their focus of bio-research from single cell studies to entire organisms, analyzing the complex interaction within whole animals. Thus, modern developmental biology is an emerging field of research, studying the dynamics of cell growth, differentiation processes and the development of organs in vivo. As living organisms grow in three dimensions these studies require imaging systems which provide - beside high resolution ­ a large workspace and field of view. [. . . ] Following the changes of life Studying movements of cancer cells in bio-medical research, investigating translocations after photo-activation, studying the growth of bio films on large implants: The creativity for future experiments will come from you, the extended freedom for new research applications is provided by Leica TCS LSI. 16 Zebra fish development video 11 Confocal technology · Highest resolution · · · · · · · True confocal point scanner Spectral detector Solid state laser 405, 488, 532, 635 nm excitation AOTF controlled Fully automated Easy to use Crystal clear 3D images ­ The benefits of true spectral confocal imaging Leica TCS LSI is equipped with a true spectral confocal scanner that provides ultimate crisp resolution. Scanning the specimen in thin optical layers and detecting the fluorescence signal point-by-point results in images free from the stray light of adjacent elements. The result ­ brilliant images at very high resolution. The World's First Super Zoom Confocal Technique for new perspectives The Confocal Principle Detector Confocal pinhole Information from each signal in all optical sections is reconstructed by intelligent software into excellent 3D images, resolving the smallest detail of the specimen's structure. To ensure high quality imaging, optimal excitation is provided by up to four solid-state lasers with 488, 532 and 635 nm lines for common dyes. The broad range of applications is extended by the 405 nm laser option for nuclear staining. Maximum signal efficiency by spectral detection Maximize the signal independent from barriers posed by fixed filters and tune freely the emission band from 430 nm to 750 nm. Cross-talk of different dyes in one sample is prevented when tuning the spectral detector precisely in 10 nm steps to the respective dye. For unrivalled detection efficiency, the Leica TCS LSI uses a Beam Splitter Laser Objective Focal plane The Spectral Principle Light beam Prism Variable Spectral Detector Collimation Optics Detector 12 prism spectral detector. Minimize bleaching and cell damage by optimizing the detection range and reduce the excitation power via Acousto-Optical Tuneable Filter (AOTF). Highly innovative Zoom freely in and out, switching between overview and smallest detail without changing the objectives. The Leica Z16 APO A 16:1 super zoom offers the largest magnification range from 0, 57x to 9, 2x, whereas the Z6 APO A ranges from 0, 57x to 3. 6x ­ at excellent optical performance. The Z-zoom systems are fully apochromatic and allow to adjust magnification continuously and parallax-free. Benefit from the motorized versions Z16 APO A and Z6 APO A: Fully software controlled, the magnification can be completely altered without touching the imaging system. To achieve precise focusing for sharpest images over a wide range of 10 mm, all motorized zooms are equipped additionally with a motorized fine focus lens. Macro and Micro: New dimensions for largest specimen Benefit from the high quality macro objectives of Leica Microsystems. A novelty in confocal imaging: the tremendous working distance (WD) of 97 mm and a field of view (FOV) of 16 mm provided by the 1x apochromatic macro objective. Additionally, classic microobjectives can be adapted to use the Leica TCS LSI as a classical confocal. Minimum training is required even for newcomers. The standardized intuitive user interface of Leica LAS AF enables you to start your work autonomously from the first button click. Ergonomic software with workflow-oriented screens guides you through your experiment from the first confocal image up to the full 3D reconstruction of your specimen. The effect: Highly efficient imaging and best results on the first shot. Additional functionality like deconvolution or spectral unmixing ensure application flexibility for the future. · · · · · · · · Minimal training effort Large workspace Variable magnification Workflow orientated Fully automated system Comfortable operation Easy sample access and manipulation Compact design Easy sample handling 15 Efficient Operation for Faster Success Workflow orientated hardware design The time for preparation, pre-selection and orientation of the specimen reduces enormously as macro and high-resolution confocal are combined in one in vivo system. [. . . ] Mouse transgenic embryo, interlimb somites Five interlimb somites of an E10. 5 mouse transgenic embryo: EpaxialMyf5 eGFP; immuno-stained for GFP-Alexa 488; embryonic muscle fibers stained with Desmin-Cy3, the nuclei are revealed with Hoechst Size from top to bottom: 3. 5 mm left, 800 µm right. Courtesy of: Aurélie Jory and Shahragim Tajbakhsh, Cellules Souches et Développement, Institut Pasteur, Paris, France and Imaging centre of IGBMC, IGBMC, Illkirch, France. Larvae, Head with nerve system, Red: Cy3, Green: Alexa 488, Eye: Photoreceptors with nerve system. Zebra fish, novocord development Danio Rerio. [. . . ]