User manual LEICA TL RCI

[. . . ] Transmitted Light Bases TL RCTM (MDG 32) TL RCITM (MDG 30) User Manual Contents Page Overviews Safety concept . . 12 Light intensity and color temperature . . 22 2 Transmitted-light bases TL RCTM and TL RCITM ­ Safety concept Dear User Thank you very much for your trust in Leica Microsystems. We hope your work with our high-quality, efficient products is both enjoyable and successful. [. . . ] Pull the upper part of the cross-stage carefully toward the user, fixing the lower part onto the transmitted-light base. Attach the cross-stage evenly to the three threaded holes. Push the cross-stage all the way back to the fence in direction of the column. Re-insert the glass plate back into the standard stage. 3. 1 Unpacking the base The base is delivered with the adapter plate attached. The selected stage (IsoProTM crossstage or standard stage 10 447 269), and the focusing drive will have to be mounted later. Insert the power cable into the mains socket (4. 2), and then connect it to a grounded outlet. TL RCTM and TL RCITM transmitted-light bases ­ Configuration 11 Operation 4. 1 Switch for adjusting the relief contrast Use the two switches (8. 1 and 8. 2) on the left side of the transmitted-light base TL RCTM/TL RCITM to actuate the two integrated flaps. The outer switch (8. 1) controls the inverted relief contrast. The inner switch (8. 2) controls the positive relief contrast. Depending on the flap position, part of the opening of the integrated Fresnel lens is covered causing different contrast effects. Phase structures typically appear as spatial, relief images ­ like hills in a positive relief contrast and as recesses in inverted relief contrast. The contrast can be increased without relief by adjusting the two diaphragms to 45°. The slot can be moved over the entire field of view and quickly switched between positive and negative relief view by precise tilting of the path-folding mirror. The dynamic effect makes it simple to distinguish phase structures from amplitude structures. Using the black rotary knob (8. 1) on the left side of the transmitted light base you can turn and push the path-folding mirror back and forth. The concave recess at the grip shows the concave side of the mirror and allows the intuitive operation without eye contact at anytime. Depending on the angle and position of the mirror the angle of light incidence changes on the specimen level so that you can move between a transmitted light bright field, through oblique illumination to dark field like illumination. The black rotary knob (8. 1) on the left side of the transmitted-light base is used: ­ To rotate the path-folding mirror from the flat to the concave side ­ For slight tilting, to steer the light beam more steeply or flatly through the object level ­ To shift the path-folding mirror (forward/ backward) Depending on the properties of the object (refractive index to environment) and your own sensitivity, it may occur that you need to operate the switches for the positive and inverted relief contrast in reverse. instead of the top switch (8. 1), the bottom switch (8. 2) controls the inverted relief contrast. Instead of the bottom switch (8. 2), the top switch (8. 1) controls the positive relief contrast. 4. 3 Operating the cross-stage IsoProTM To move the stage in X direction rotate the outer knob (7. 1) To move the stage in Y direction rotate the inner ring around the knob (7. 2) 4. 4 Light intensity and color temperature 4. 4. 1 Transmitted-light base TL RCTM Please observe the user manual and in particular the safety regulations of the manufacturers of the light guide and cold light source. Turn on the cold light source according to the manufacturer's user manual and connect, activate, and adjust the light intensity. 4. 2 Path-folding mirror The built-in path-folding mirror features a flat and a concave side and can be rotated and moved. The concave side was specifically designed for the optical features of objectives with high 12 TL RCTM and TL RCITM transmitted-light bases ­ Operation 1 Fig. 8 1 2 1 Switch to control the inverted relief contrast 2 Switch to control the positive relief contrast 3 Path-folding mirror 1 Potentiometer Control of IsoColTM illumination intensity 2 Potentiometer Control of dye temperature 4. 4. 2 TL RCITM transmitted-light base The TL RCITM transmitted-light base has two electronic potentiometers to control the dye intensity (9. 1), and dye temperature (9. 2) separately. Adjust the dye intensity using the front potentiometer (9. 1) to match your requirements. [. . . ] You are using a precision instrument with many performance features. As appropriate for such instruments we guarantee the quality of our products. This guarantee covers all manufacturing and material defects of the original equipment, but not any damages that are the result of inappropriate use or negligence. Please handle your valuable optical instrument with appropriate care. [. . . ]