User manual ZEISS AXIOPHOT INSTALLATION

[. . . ] Violations will entail an obligation to pay compensation. All rights reserved in the event of granting of patents or registration of an utility model. Issued by: Carl Zeiss Microscopy D-07740 Jena Phone: ++49-36 41 64-16 16 Telefax: ++49-36 41 64-31 44 E-mail: micro@zeiss. de Internet: www. zeiss. de/micro Number of this operating manual: B 40-015 e Date of issue: 06/99 II B 40-015 e 06/99 Axiolab A Carl Zeiss CONTENTS Page INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I Title Page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I Copyright . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] plug-in diameter. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30 mm B 40-015 e 06/99 1-23 Carl Zeiss Axiolab A 1-24 06/99 B 40-015 e Axiolab A Carl Zeiss START-UP Contents 2 2. 1 2. 2 2. 3 2. 3. 1 2. 3. 2 2. 4 2. 5 2. 6 2. 6. 1 2. 7 2. 8 2. 9 Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3 Unpacking the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3 Screw in Objectives. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3 Insertion of Eyepieces . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4 Insertion of Eyepiece Reticle . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-4 Compensation of Ametropia when Eyepiece Reticles are used . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5 Setting of Interpupillary Distance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5 Attachment of Reflected-Light Halogen Illuminator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6 Retrofit the Transmitted-Light Halogen Illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6 Switch on the Transmitted-Light Halogen Illuminator. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7 Equipment of Filter Slider . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-7 Set the Luminous-Field Diaphragm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8 Connecting the Instrument to the Line . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-8 B 40-015 e 06/99 2-1 Carl Zeiss Axiolab A 2-2 B 40-015 e 06/99 Axiolab A Carl Zeiss 2 START-UP The three versions of the Axiolab A reflected-light microscope, including accessories, are supplied in standard packaging. We would recommend you to keep the transport cases for storage or return of the instrument to the manufacturer. 2. 1 Unpacking the Instrument · Remove the microscope from the transport case and place it on the mat (2-1/1) provided on the worktable. · Remove the plastic sleeve (2-1/4) from the instrument. · Remove the adhesive strips which secure the various controls during transport. · Leave dust protection and technique sliders (2-1/2, 5, 6) inserted in the reflected-light illuminator H (2-1/3) in the instrument. Fig. 2-1 Unpacking the instrument 2. 2 Screw in Objectives · Remove the dust caps (2-2/2) according to the number of objectives and screw the objectives into the nosepiece (2-2/1) clockwise one by one, starting with the lowest magnification (2-2/3). The dust caps should remain on those nosepiece eyes which are not required. Fig. 2-2 Screw in objectives B 40-015 e 06/99 2-3 Carl Zeiss Axiolab A 2. 3 Insertion of Eyepieces · Remove both protection caps from the binocular tube. · Insert the fixed eyepiece, e. g. E-PL 10×/20 Br. , in one tube and the focusing eyepiece E-PL 10×/20 Br. Furthermore, this restriction of the field of view enables reduction of the false light portion of weakly reflecting reflected-light specimens. Fig. 2-6 Setting the luminous-field diaphragm 2. 9 Connecting the Instrument to the Line The microscope can be operated using line voltages of 100 - 240 V without conversion · Connect the line cable (2-7/1) with connector to the instrument socket (2-7/3) and connect the earth-contact plug to the line. Switch on the instrument via the on/off switch (2-7/2) on the left-hand side of the instrument. The green LED integrated into the on/off switch lights up to indicate that the machine is ready for operation (switch in , , I" position). The integrated halogen lamp 6 V, 30 W must also be on. · · Fig. 2-7 Connecting the instrument to the line The change of fuses is described in section 4. 2. 2-8 B 40-015 e 06/99 Axiolab A Carl Zeiss OPERATION Contents 3 3. 1 3. 2 3. 2. 1 3. 2. 2 3. 2. 3 3. 2. 4 3. 2. 5 3. 3 3. 3. 1 3. 4 3. 5 3. 5. 1 3. 5. 2 3. 6 Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Switch on the Instrument. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Illumination and Contrasting Techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4 Setting of Reflected-Light Brightfield . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4 Setting of Reflected-Light Polarization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5 Setting of Transmitted-Light Polarization with extended Polarization Equipment. . . . . . . . . . . . . . . . 3-5 Setting of Epi-Fluorescence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13 Setting of Transmitted-Light Brightfield (KÖHLER Illumination) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14 Attachment of Microscope Stages and Specimen Holders . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-15 Attachment of Pol Rotary Stage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16 Use of Polished Section Attachment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-17 Photomicrography and Videomicroscopy . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] · Check line cable (4-1/1) and replace it, if required. · Use 2. 0 mm watchmaker's screwdriver to press the two springs at the side of the fuse holder (4-1/2) to the inside via the groove and remove fuse holder. · Check fuse inserts (4-1/3) and replace defective fuses with new ones. Make sure to use only T 0. 8 A; 250 V; 5 × 20 mm fuses! Fig. [. . . ]