User manual ZEISS AXIOPHOT 2

[. . . ] Axioplan 2 imaging and Axiophot 2 Universal Microscopes Operating Manual 2 1 Knowledge of this manual is required for the operation of the instrument. Please therefore familiarize yourself with its contents and pay special regard to the sections dealing with the safe handling of the instrument. Unless expressly authorized, forwarding and duplication of this document, utilization and communication of its contents are not permitted. Violations will entail an obligation to pay compensation. All rights reserved in the event of granting of patents or registration of a utility model. [. . . ] Adjust the specimen (use a thick specimen mount). Image the luminous field diaphragm (close it until it be comes visible). Move the condenser slightly upwards (diaphragm image becomes unsharp). Press stop screw (8) upwards and tighten it again. Your specimen can now no longer be touched by the con denser. Achromatic aplanatic condenser system (Fig. 34) Depending on the version, the achromatic aplanatic universal condenser (1) is equipped with: - Fold out front lens - Turret disk for: brightfield - without or with DIC I, II, III darkfield - D phase contrast - Ph 1, Ph 2, Ph 3 interference contrast - DIC I, II, III - aperture diaphragm (iris stop) The front lens is folded in / out using lever (2). Turning the turret wheel (4) swings the brightfield insert and the contrast stops in the beam path. The abbreviation of the set turret position (e. g. D) is displayed in one of the windows (5). Slider (3) opens and closes the aperture diaphragm. 5 4 3 2 1 2 3 4 5 Universal condenser Lever to swing in/out the front lens Sliding knob for aperture diaphragm Window for turret position Turret wheel to swing in the brightfield insert or the contrast stops Condenser modules Fig. 34 13 54 B 40 042 e 09/99 Microscope Components Changing the phase stops or darkfield stops in the universal condenser (if required) (Fig. 35) 1 1 1 2 3 4 1 2 3 4 Fig. 35 Centering screws Cover on the underside Stop Retainer ring Changing the phase stop Tighten both centering screws (1) until stop using SW 1. 5 ball headed screwdriver. To loosen the cover on the condenser underside (2), loosen both grub screws using SW 2 screwdriver and remove the cover. Position turret disk with stop to be changed in the exchange opening and hold it on the knurled ring, since no click stop is effective in this position. Use mounting device contained in tool set to unscrew retainer ring (4) from the condenser underside (2) and allow phase stop (3) to slide out. To insert the new stop, turn condenser round, insert the new stop, and tighten retainer ring. The shiny glass underside of the stop must point downwards when the condenser is inserted. Loosen both centering screws and recenter stop using the centering telescope. Make sure that the correct label is visible on the knurled ring of the turret when the unit is swung into the beam path. Note: Phase or darkfield stops may only be inserted in centerable openings, DIC prisms only in openings with an iris stop. Changing the DIC prisms in the universal condenser (Fig. 36) The positions of the DIC prisms are marked with I, II, III. 1 1 2 3 5 4 1 2 3 4 5 Fig. [. . . ] 15 Compensation of reciprocity failure, . 55 Differential Interference Contrast, . 62 Light exit of transmitted light equipment, . 12 B 40 042 e 09/99 87 Index M Manual focusing drive, . [. . . ]