User manual ZEISS SV11

[. . . ] Violations will entail an obligation to pay compensation. All rights reserved in the event of granting of patents or registration of a utility model. Issued by: Carl Zeiss Mikroskopie D-07740 Jena Telephone: (**49) 03641 / 64-1616 Telefax: (**49) 03641 / 64-3144 Internet: mikro@zeiss. de http://www. zeiss. de Number of this manual: B 40-029 e Date of issue: 01/98 II B 40-029 e 01/98 Standard 25 ICS CONTENTS Page INTRODUCTION. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I Title page . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I Copyright. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] (2- 3/3) in the right tube and the focusing eyepiece Pl 10x/18 Br. The focusing eyepiece is used to compensate for ametropia of the eyes. Fig. 2-3 Insertion of eyepieces 2. 4. 1 Insertion of eyepiece reticle The eyepieces Pl 10x/18 Br. are intended for use with eyepiece reticles (see overview under 1. 6). The slight image shift caused by the additional path through the glass is taken into account on the diopter scale by the fact that the zero point position is indicated not by the white dot W (24/W) but by the red dot R (2-4/R). The eyepiece reticles (2-4/1) have been adhered to screw-in mounts (2-4/2) to allow easy replacement. Fig. 2-4 Insertion of eyepiece reticle To replace a reticle, unscrew the screw-on mount or the part containing the eyepiece reticle and replace it with the required one. If eyepiece reticles are inserted into the unscrewed mount by the customer, attention must be paid to the labelling being visible the right way up after insertion. 2-4 B 40-029 e 01/98 Standard 25 ICS 2. 4. 2 Compensation of ametropia when eyepiece reticles are used The correct use of an eyepiece reticle requires two focusing eyepieces, e. g. foc. , to enable compensation of ametropia. Use the eyelens of the focusing eyepiece to focus on the line figure of the eyepiece reticle; focus on the edge of the field of view if no eyepiece reticle is used. Focus on the microscope image of a specimen via the focusing drive by looking through the eyepiece with reticle. When the image and the eypiece reticle are in focus in the above eyepiece, focus the image for the second eye via the focusing eyelens of the second eyepiece. The position of the focusing drive on the stand must not be changed. 2. 5 Setting of interpupillary distance and viewing height The eyepiece distance is matched to the individual interpupillary distance by swinging the eyepiece tubes symmetrically towards one another. The viewing height can be increased (2-5/A) or reduced (2-5/B) by turning the entire binocular tube through 180°. Fig. 2-5 Setting the interpupillary distance and viewing height of the binocular tube B 40-029 e 01/98 2-5 Standard 25 ICS 2. 6 Attachment of specimen holder to mechanical stage 75x30 R The Standard 25 ICS features the mechanical stage 75x30 R with ceramic coating (2-6/6). The specimen is precisely moved in x and y using the user-friendly coaxial drive (2-6/4). The x/y gradation on the stage surface (2-6/5) with the two vernier scales helps to relocate certain specimen spots. 3-16 B 40-029 e 01/98 3-1 Standard 25 ICS 3-2 B 40-029 e 01/98 Standard 25 ICS 3 3. 1 OPERATION Switch on the instrument Switch on the instrument via the on/off switch (3-1/3) on the instrument rear. The green LED (3-1/2) must light up. Set the required brightness via the "Brightness" control (3-1/1). Fig. 3-1 Switch on the instrument Depending on the application, place one or several of the following dia. 32 filters (3-2/1) on the dustprotection glass of the luminous-field diaphragm: interference wide-band filter, green, 32x4, for contrast enhancement in b/w photography of stained sections and for phase contrast; CB 3 conversion filter, 32x2, to generate the correct color temperature of 3200 K when artificial light color reversal film is used. CB 3, 32x2, and CB 12, 32x2, conversion filters for use with daylight color film Fig. 3-2 Insertion of filters The Standard 25 ICS is supplied with factory-aligned illumination. The illumination need not be adjusted even when the lamp is exchanged by the customers themselves. B 40-029 e 01/98 3-3 Standard 25 ICS 3. 2 Setting of transmitted-light brightfield for KÖHLER illumination As described in chapter 2, the microscope is ready for operation and switched on according to section 3. 1. If the Ph 2 phase stop carrier (3-3/4) is available on condenser 0. 9 Z (3-3/6), it should be swung out of the optical beam path for microscopy in transmitted-light brightfield. Furthermore, the following basic settings of the microscope are required: First, place a high-contrast specimen with 0. 17 mm cover slip on the mechanical stage 75x30 R (3-3/8). Swing 10x objective into beam path via knurled ring (3-3/9) of the nosepiece. Look throught the fixed eyepiece of the Fig. 3-3 Setting of brightfield binocular tube (3-3/1) first and focus on the object via the focusing drive (3-3/2). Then set the focus for the other eye by turning the eyelens of the focusing eyepiece. The eyepiece distance is matched to the individual interpupillary distance by swinging the eyepiece tubes symmetrically towards one another. Set the interpupillary distance in such a way that you can see a sharply limited field of view with both eyes. The viewing height can be increased (3-4/A) or reduced (3-4/B) by turning the entire binocular tube through 180°. Fig. [. . . ] Ring is not set to the correct cover slip thickness Set the corr. ring to the correct thickness, see page 1-6. Dirt or dust on the optical surfaces Clean the appropriate of objectives, eyepieces, components, see page 4-3 condensers or filters Image aberration Condenser not set correctly. Nosepiece not correctly switched to stop position Specimen is not fixed on the mechanical stage Great focus differences after objective change Focusing eyepieces are not set correctly. Set condenser correctly, see pages 3-4, 3-5 Correctly click-stop nosepiece Correctly set specimen in specimen holder and fix it. [. . . ]