User manual ZEISS UNIVERSAL R MICROSCOPE

[. . . ] Violations will entail an obligation to pay compensation. All rights reserved in the event of granting of patents or registration of a utility model. Issued by: Carl Zeiss Microscopy D-07740 Jena Phone: ++49-36 41 64-1616 Telefax: ++49-36 41 64-3144 E-mail: micro@zeiss. de Internet: www. zeiss. de/micro Number of this manual: B 40-046 e Date of issue: 31. 03. 1999 0-2 B 40-046 e 03/99 Photomicrography using 35 mm SLR cameras INTRODUCTION Contents Carl Zeiss CONTENTS Page INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0-1 Title Page. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0-1 Copyright . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0-2 Table of Contents . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 0-3 Notes on Instrument Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] 2-4 Attachment of 35 mm SLR cameras with objective f' = 63mm to camera ports 44 mm and 60mm 2-6 B 40-046 e 03/99 Photomicrography with 35 mm SLR cameras START-UP Attachment of 35 mm SLR cameras Carl Zeiss 2. 3. 3 Attachment of 35 mm SLR Cameras with 2. 5× / T2 Adapter to Front Port of Axiovert 25 C / CFL / CA · Remove dust cover or lens from the SLR camera housing. · Attach camera-specific T2 adapter (2-5/3) to the relevant SLR camera housing (2-5/2). · Remove dust covers from 2. 5× / T2 camera adapter for Axiovert 25 (2-5/4). · Screw thread of 2. 5× / T2 adapter for Axiovert (2-5/4) in the T2 adapter (2-5/3) attached to the SLR camera housing (2-5/2). · Remove dust cover from the front camera port of the Axiovert 25 (2-5/6). · Push 2. 5× / T2 camera adapter for Axiovert 25 (2-5/4) with SLR camera housing in the front camera port of the Axiovert 25 (2-5/6) until stop. · Align SLR camera horizontally and tighten clamping screws (2-5/7) using the ball-headed screwdriver (2-5/10). · Fix cable release (2-5/1), if required. Fig. 2-5 Attachment of 35 mm SLR cameras with camera adapter 2. 5× / T2 to front-port of Axiovert 25 C / CFL / CA B 40-046 e 03/99 2-7 Carl Zeiss START-UP Attachment of 35 mm SLR cameras Photomicrography with 35 mm SLR cameras 2. 3. 4 Attachment of 35 mm SLR Cameras to Front Camera Port of Axiovert 35 / 35 M / 135 / 135 M The front camera port (2-6/5) of the Axiovert 35 / 35 M / 135 / 135 M is a T2 adapter with camera factor 2. 5× attached to the stand. · Remove dust cover or lens from the SLR camera housing (2-6/2). · Remove dust covers from the front camera ports (2-6/5) of the Axiovert 35 / 35 M/135 / 135 M. All the photographs with exposure times the border exposure time are unblurred. 2-10 B 40-046 e 03/99 Photomicrography with 35 mm SLR cameras OPERATION Contents Carl Zeiss OPERATION Contents 3 3. 1 3. 2 3. 2. 1 3. 2. 2 3. 2. 3 3. 2. 4 3. 2. 5 3. 2. 6 3. 2. 7 3. 2. 8 3. 2. 8. 1 3. 2. 8. 2 3. 2. 8. 3 3. 2. 8. 4 3. 2. 8. 5 3. 3 OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2 Adaptation and Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2 Photomicrography with 35 mm SLR Cameras . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Photography in a Few Steps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Setting the Color Temperature to 3200 K and Setting the Image Brightness. . . . . . . . . . . . . . . . . . . . . . 3-4 Selecting the Film . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4 Exposure Correction Depending on the Microscopy Technique. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5 Setting and Focusing of Image Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6 Exposure Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7 Trigger the Exposure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10 Further Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11 Avoid Blurs . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11 Color Balance in Color Reversal Films. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11 Darkfield and Fluorescence Photography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13 Computing the Magnification . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14 Further Filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14 Using Digital Cameras. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Use of bulk film . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5 Measuring range of 35 mm SLR camera, ISO 100 / 21°, camera lens f / 1. 4 with apertures k = 1. 4 - 16 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8 Measuring range of 35 mm SLR camera with T2-Adapter, ISO 100 / 21° . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9 B 40-046 e 03/99 3-1 Carl Zeiss OPERATION Adaptation and functions Photomicrography with 35 mm SLR cameras 3 3. 1 OPERATION Adaptation and Functions The adaptation components for 35 mm SLR cameras for photomicrography are simple screwing and clamping adapters which are described in chapter 2, Start-up. The functions important for photomicrography with 35 mm SLR cameras (e. g. exposure programs, film insertion) are described in the operating instructions of the relevant SLR camera. The T2 adapters suitable for the used 35 mm SLR cameras are attached and removed in the same way as camera lenses. [. . . ] of filters, photo reticles) Deutsches Institut für Normung (German standards association) coding system for the storage of electronically legible information (e. g. film speed) Extra-Plan (eyepiece model) Exposure Value (of light) camera lens with focal length f with aperture ratio 1 : 1. 4 focal length on the image side focal length of objective fluorescence focusing green Infinity Color-Corrected System Inverted Microscope International Organization for Standardization stop value compensation Kompens-Plan (eypiece model) reference viewing distance 250 mm metric thread, magenta microscope camera magnification of the image on 35 mm film Plan red Single Lens Reflex (camera) Sonder-Plan (photo eyepiece, projection lens for photomicrography) A-2 B 40-046 e 03/99 Photomicrography with 35 mm SLR cameras ANNEX List of abbreviations Carl Zeiss SW T2-Adapter T-function Tmax Tmin VObj Veyep. VZ W-PL Y wrench opening standard adapter for 35 mm cameras Time function (shutter remains open after first press and closes only after second press, see B-function) maximum exposure time minimum exposure time magnification of the objective magnification of the eyepiece intermediate magnification Weitfeld-Plan (wide-angle eyepiece model)) Yellow Physical and Technical Data °C hPa K kg m mm s V Centigrade Hecto-Pascal Kelvin kilogram meter millimeter second Volt B 40-046 e 03/99 A-3 Carl Zeiss ANNEX List of key words Photomicrography with 35 mm SLR cameras List of Key Words Page A adjusting aid. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6 ambient conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 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