User manual BIOPTECHS DELTAT4CONTROLLER

[. . . ] Delta T4 Culture Dish System Instructions Caution: Read Carefully Before Use! The System is comprised of three main components: a temperature controller, a stage adapter, and the Delta T Dish. It utilizes a new and improved technology that is much faster and more accurate than traditional peripheral stage warmers. The controller has a fast learning curve to anticipate changes in the dish temperature due to perfusion or surface evaporation while regulating temperature and compensating for entropy. The fast response capability of this system enables control features previously not possible with conventional peripheral systems. [. . . ] Temp Out is a voltage output direct from two instrument amplifiers reading the thermistors from channel A and B. The output from this socket is temperature /10. Left pin is channel A, center pin is channel B, and right pin is ground. Remote Setpoint is an analog input that allows the user to set the controllers setpoint from an external DC source. A DC voltage can be applied to the tip of this jack equal to temperature /10. When the jack is inserted into the controller it will automatically switch to the jacks value. When removed it will default to the setting on the front of the controller. Use for programming or cycling operating temperatures. Fixing Adjustment allows the user to adjust the voltage sent to the dish when the Fixing Switch is depressed. Fixing Switch is provided so that cells can be fixed on the stage of the microscope. It can also be used for heat shock experiments. The switch is recessed so that it is not actuated by mistake. This switch should be used when you intentionally want to overheat your cells. It provides a nearly instantaneous heat shock to the dish. TTL is a BNC connection used to change the mode of operation of the controller from a remote source such as a computer or slave from a shutter trigger. The default condition of the controller is dynamic control. To change to Imaging mode, apply a TTL logic high and hold to this BNC. The controller will remain in Imaging mode as long as the TTL condition remains high as indicated by the LED on the front panel. 3560 Beck Road · Butler, PA 16002 · Voice: 724-282-7145 · Fax: 724-282-0745 · E-mail: info@bioptechs. com · Web: www. bioptechs. com Instructions: 1. 2. Delta T4 Controller Operation 3. 7. 8. 9. Without any peripherals plugged into the controller, turn the unit on using the main on-off switch located on the rear panel adjacent to the power cord. The temperature display will read a number between -3. 7 and -4. 2. Press and hold the black button labeled "CALIBRATE" and make sure the display reads 25. 0°C. [. . . ] The sensitivity of the controller, 0. 1°C, coupled with the speed and intensity of the controller's response, may cause the coverslip to flex a detectable amount during narrow depth of field imaging. The controller is equipped with a circuit that bypasses the dynamic temperature control mode and permits you to temporarily send a constant DC current to the dish to eliminate the possibility of an abrupt change of energy occurring as might happen in dynamic mode. This prevents Zaxis displacement of the specimen during imaging. The controller can be switched from its default Dynamic control mode to Imaging mode by several means: 1. [. . . ]