User manual BIOPTECHS FCS2

[. . . ] This button can be pressed at any time and has no effect on the temperature regulation functions of the controller. Display Lights indicate the source of the value displayed on the numeric display. Setpoint is adjusted by a potentiometer accessible with the 2. 5mm screwdriver provided. To adjust the setpoint set the numeric display to Setpoint, then adjust the potentiometer to the desired value. [. . . ] If adjustment is necessary, turn the corresponding screws in the adjustment holes with the enclosed screwdriver. With the chamber loaded, plug the chamber into the controller. DO NOT plug the chamber into the controller unless it is either loaded or both slide contact wires are restricted from coming in contact with the base. When the controller is turned on and the display switch is set to "Slide" or "Chamber" it will read the ambient temperature, then slowly begin to rise until it reaches setpoint temperature. We recommend leaving the display switch in the "Slide" position because it is the closest representation of the cell temperature. Special Notes: There is a safety cut-off circuit that protects the cells if the microaqueduct drops below 0. 9°C from the setpoint after reaching setpoint minus 0. 9°C. Only during a cold start will the safety cut-off circuit ignore the out-of-range condition. This safety circuit will shut off power to the slide circuit only and sound the alarm. If the alarm sounds, the user should correct the problem and press the red reset button located next to the red LED. 3560 Beck Road · Butler, PA 16002 · Voice: 724-282-7145 · Fax: 724-282-0745 · E-mail: info@bioptechs. com · Web: www. bioptechs. com The alarm may sound as a result of the following conditions: · Perfusion rate exceeds the rate which temperature stability can be maintained. To correct, press the red reset button as soon as the alarm sounds. This will reset the logic back to cold start up mode. Setpoint temperature will return within 15-20 seconds. · An interruption in AC line power may cause the logic levels to change state. · Physical displacement of the thermistor from the microaqueduct surface. Note: The thermistor that regulates the chamber frame is sealed internally eliminating accidental displacement. Therefore, there is no need for an alarm on the chamber frame circuit. Chamber Loading Your chamber has been inspected prior to packaging and is ready to use when you receive it. The chamber is shipped assembled and should be opened using the following procedure. Rotate the large knurled ring in the base of the chamber clockwise until it stops. This will release and disengage the chamber top. Hold the white portion of the chamber while simultaneously (using thumb and forefinger) rotating CCW the two smaller knurled nuts located on either side of the black electrical contact unit. This will remove the black portion which contains sensitive electronics so that they can remain dry. You now have the basic components of the chamber separated. Continue with the following directions: Directions for Closed System Use: (see drawing for part identification) · Attach perfusion tubing to the inlet and outlet ports of the chamber. [. . . ] High Numeric Aperture Use: If you are using high numeric aperture (immersion) objectives with high N. A. dishes, it will be necessary to regulate the temperature of the objective as well. This is due to the fact that the optical coupling medium, oil, glycerin or water also has a thermal coupling effect. Objective Heater and an Objective Heater Controller will be necessary for uniform temperature across the field. [. . . ]