User manual BIOPTECHS FCS2 OPEN MODE

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Manual abstract: user guide BIOPTECHS FCS2 OPEN MODE

Detailed instructions for use are in the User's Guide.

 [. . . ] FCS2 Open Mode Adapter Instructions
The open mode adapter resembles the standard top portion of a FCS2 except that it does not have perfusion ports and provides much wider vertical access to cells on the 40mm coverslip.  [. . . ] This will release and disengage the closed system chamber top. You now have the basic components of the chamber separated. Continue with the following directions. 

Instructions: (see drawing above for part identification)
Note: This assembly should take place in a biological safety cabinet and should not require more than 15 seconds to compete. The gasket and open mode top should be autoclaved before loading cells. The base of the FCS2 should be wiped (not immersed) with 70% ETOH (do not autoclave the metal base). Place the 40mm coverslip containing cells (cell side up) into the aperture of the FCS2 base. Place the 1mm thick x 40mm OD x 30mm ID gasket previously autoclaved onto the coverslip. Place the Open Mode FCS2 Adapter on top of the gasket coverslip combination then turn the knurled ring on the base CCW to tighten and seal these components.  [. . . ] Follow the FCS2 instructions to set the desired temperature. 

3560 Beck Road · Butler,  PA 16002 · Voice: 724-282-7145 · Fax: 724-282-0745 · E-mail: info@bioptechs. com · Web: www. bioptechs. com [. . . ]

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