User manual FLUORESCENCE RS-DAQ

[. . . ] Model RS-DAQ Instrument Controller User Manual Version 1. 0 C&L Instruments, Inc. 314 Scout Lane Hummelstown, PA 17036 Phone: 717-566-3642 Fax: 717-566-3643 E-mail: support@fluorescence. com Web site: http://www. fluorescence. com Table of Contents Chapter 1: Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2 Chapter 2: Setup . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Cable Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3 Chapter 3: Using the Controller . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4 Power on considerations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] 1Spin 2Fix 3Mix Sel EM: If Mix is selected, however, then the Mixed mode is automatically selected for the emission wheel. This selection is automatic since both the excitation and emission filter wheels must both operate in the Mixed mode. Further details of the Mixed mode are discussed below. If the Spin or Fixed mode is selected for the excitation or emission filter wheel, the user is prompted to enter the spin aperture time and the fixed wheel position. The prompts for these entries are: SpinApTime: Fix ExFilter: Fix EmFilter: The user is prompted for the appropriate entry for the excitation wheel first, followed by the entry for the emission wheel. The spin aperture time is the duration of time, in milliseconds, that the filter wheel is in the light path while the filter wheel is spinning. This speed also determines the rate of data collection when the RS-232 Controller is used in conjunction with data acquisition software provided by C&L Instruments. The valid entries for Fix ExFilter: and Fix EmFilter: can be either 1 to 4 or 1 to 8, depending on the type of filter wheel (either 4 or 8 position) entered in response to the earlier prompts. If the excitation and emission filter wheels are different types, then they cannot be both programmed to spin simultaneously. For this reason, the user is encouraged to use the same type of filter wheel (either 4 or 8 position) in both the excitation and emission optics. If the spin mode is selected for both filter wheels after different types of filter wheels have been entered, the following error message will be displayed when the Controller attempts to initialize the hardware. Diff ExEmFilters Press CLR If valid choices of Fixed and Spin are entered for the excitation and emission wheels, the user is then prompted to select the state of the Neutral Density wheel. This setting determines the output intensity of the illumination source. This feature is designed for when the Controller is used with the C&L Instruments Model S48D Illumination Source. Fix NdPos: Valid entries are between 1 and 100. An entry of 100 indicates full illumination (i. e. , 100%) and an entry of 1 indicates minimal illumination. RS-DAQ Instrument Controller User Manual (version 1. 0) Page 9 Chapter 4: Programming the Controller After the filter wheel modes and the Neutral Density wheel are programmed, the user is prompted to select the state of the excitation and emission shutters. 1 Close 2 Open ExShutter: and 1 Close 2 Open EmShutter: Select the initial state of the excitation and emission shutters. These commands work together with the two toggle switches on the front panel that can also be used to control the shutters. If the shutter is programmed to be closed (choice 1), then the state of the shutter can also be controlled with the toggle switches on the front panel. If the shutter is programmed to be initially open, the toggle switches on the front panel are disabled. In other words, either the panel or the toggle switch can be used to open the shutter, but they both have to be set to the closed position to close the shutter. If the Mixed mode is selected, the programming sequence is slightly different from when either the Spin or Fixed mode is selected. [. . . ] Optics, including lenses and heat filters are subject to these limited warranties for thirty (30) days. The neutral density filter, if provided, is not covered by this warranty. All repairs must be made by C&L and Customer must return the Instrument and/or component to C&L, at customer's cost, for repair. Return of the instrument for repair must be preapproved by C&L. [. . . ]