User manual HANNA INSTRUMENTS HI 83740

[. . . ] Instruction Manual HI 83740 COPPER ISM for wine analysis MAN83740R3 04/06 w w w . h a n n a i n s t . c o m www. hannainst. com This Instrument is in Compliance with the CE Directives Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst. com. [. . . ] It is important to check the copper content both in must and in wine, because at levels above 9 mg/L copper becomes a metabolic toxin that inhibits or delays alcoholic fermentation, and concentrations exceeding 1 mg/L may be sensorial detected and should be avoided. Other copper related problems can be manifested as formation of white haze (in white wines) and later as a reddish-brown amorphous precipitate. This precipitated `casse' develops only under the strongly reducing conditions found in bottled wines. It has been found that this casse is a mixture of copper compounds and proteins. Factors favouring and inhibiting copper casse formulation in wine Necessary conditions for copper casse formation strong reducing conditions low iron concentrations high protein levels light and heat Preventive Measures copper levels at less than 0. 3 mg/L limit SO2 addition cold-stabilize and bentonite fine to reduce proteins in white wine ACCESSORIES REAGENT SETS HI 83740-20 Copper reagent set for wine (20 tests) HI 83742-25 Color Reagent Set for wine (Wine Solvent-1) OTHER ACCESSORIES HI 740027P 1. 5V AA batteries (10 pcs) HI 731318 Tissue for wiping cuvets (4 pcs) HI 731321 Glass cuvets (4 pcs) HI 731325W Caps for cuvets (4 pcs) HI 93703-50 Cuvets cleaning solution (230 mL) HI 740231 20 mL glass cylinder with caps (2 pcs) 4 17 · Insert the zero (cuvet #1) into the holder and close the lit. #1 (Zero) · Press ZERO and "----" will blink on the display. · After a few seconds the display will show "-0. 0-". The meter is now zeroed and ready for measurement Note: If the "L Lo" (Low Light) message appears, the sample must be diluted. See "General tips for an accurate measurement" (page 12). · Remove the cuvet from the instrument. · Insert the reacted sample (cuvet #2) into the holder and close the lid. · Press READ and the display will show "----" during measurement. · The instrument directly displays concentration in mg/L (ppm) of copper on the Liquid Crystal Display. Range Resolution Precision Light Source Light Detector Method Environment Battery Type Auto-Shut off Dimensions Weight SPECIFICATIONS 0. 00-1. 50 mg/L 0. 05 mg/L SD ±0. 05 mg/L @ 0. 50 mg/L Tungsten lamp with narrow band interference filter @ 560 nm Silicon Photocell The reaction between Copper and the reagents causes a purple tint in the sample. 0 to 50°C (32 to 122°F); max 95% RH non-condensing 4 x 1, 5 volt AA batteries / 12 to 20 VDC through voltage adapter After 15' of non-use in measurement mode. 225 x 85 x 80 mm (8. 7 x 3. 3 x 3. 1") 500 g (17, 6 oz. ). Description Copper Reagent A Copper Reagent B Copper Reagent C Copper Reagent D Quantity/test 5 mL 5 mL 2 x 4 spoons 2 x 4 spoons #2 (Sample) REQUIRED REAGENTS Code HI 83740A-0 HI 83740B-0 HI 83740C-0 HI 83740D-0 PRECISION AND ACCURACY Precision is how closely repeated measurements agree with each other. Precision is usually expressed as standard deviation (SD). Accuracy is defined as the nearness of a test result to the true value. Although good precision suggests good accuracy, precise results can be inaccurate. In a laboratory using a standard solution of 0. 50 mg/L copper and a representative lot of reagent, an operator obtained with a single instrument a standard deviation of 0. 05 mg/L. 5 Note: If the copper concentration exceeds 1. 50 ppm or if the sample is very turbid or dark red colored, it is recommended to dilute the sample 10 times with HI 83742-1 Wine Solvent-1 and repeat the complete measurement procedure starting from the beginning, taking 15 mL of diluted wine sample in vial #1 for zero-sampling and 15 mL in vial #2 for reading. In this case the displayed value needs to be multiplied by 10 to compensate for dilution. 16 PRINCIPLE OF OPERATION Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and matter. [. . . ] The instrument is zeroed and a measurement can be made. · Read the cuvets immediately after extraction. In case of unstable readings or for more accurate results, let the reacted cuvets stand for at least 4 hours, allowing complete separation of the formed emulsion. · Before taking a measurement, verify that no air bubbles or water drops are attached to the walls of the glass cuvet. Indicates that the meter is making a measurement. Batteries voltage is getting low and the batteries need to be replaced. [. . . ]