User manual HANNA INSTRUMENTS HI 83746

[. . . ] Instruction Manual HI 83746 REDUCING SUGARS ISM for wine analysis MAN83746R2 03/06 w w w . h a n n a i n s t . c o m www. hannainst. com This Instrument is in Compliance with the CE Directives 20 1 Dear Customer, Thank you for choosing a Hanna product. This manual will provide you with the necessary information for the correct use of the instrument. Please read it carefully before using the meter. If you need additional technical information, do not hesitate to e-mail us at tech@hannainst. com. [. . . ] For a correct use of the reactor follow the Reactor Instruction Manual. Use of the optional HI 740217 safety shield is recommended. · Remove the cap from two vials HI 83746A-0 Fehling Solution A. SPECIFICATIONS Range Resolution Precision Light Source Light Detector Method Environment Battery Type Dimensions Weight 0. 00-50. 00 g/L 0. 25 g/L ±0. 50 @ 10. 00 g/L Tungsten lamp with narrow band interference filter @ 610 nm Silicon Photocell Fehling Method 0 to 50°C (32 to 122°F); max 95% RH non-condensing 4 x 1, 5 volt AA batteries / 12 to 20 VDC through voltage adapter 224 x 87 x 77 mm (8. 7 x 3. 3 x 3. 1") 512 g (17, 6 oz. ) 1 mL 1 mL · Use the HI 731341 1000 µL automatic pipette to add exactly 1 mL (=1000 µL) of HI 83746B-0 Fehling Solution B to each vial. For a correct use of the automatic pipette please follow the related Instruction Sheet. wine sample 1 mL deionized water 1 mL REQUIRED REAGENTS Code Description HI 83746A-0 Fehling solution A HI 83746B-0 Fehling solution B OPTIONAL REAGENTS Code HI 93703-59 Quantity/test 1 vial 1 mL · Use the HI 731341 1000 µL automatic pipette to add exactly 1 mL (=1000 µL) of wine sample to one vial (Sample) and 1 mL of deionized water to the other vial (Blank). Description Charcoal Quantity/test 2 spoons Sample Blank PRECISION AND ACCURACY · Replace the cap and invert the vials several times to mix. Wipe the vials thoroughly with a lint-free cloth. · Proceed with instructions on page 14 and 15. · The concentration displayed on the LCD must then be divided by 4 to obtain the correct value in g/L (ppt) of Reducing Sugar. Precision is how closely repeated measurements agree with each other. Precision is usually expressed as standard deviation (SD). Accuracy is defined as the nearness of a test result to the true value. Although good precision suggests good accuracy, precise results can be inaccurate. In a laboratory using a standard solution of 10. 00 g/L of Reducing Sugars and a representative lot of reagent, an operator obtained with a single instrument a standard deviation of ±0. 50 g/L. 5 16 PRINCIPLE OF OPERATION Absorption of Light is a typical phenomenon of interaction between electromagnetic radiation and matter. When a light beam crosses a substance, some of the radiation may be absorbed by atoms, molecules or crystal lattices. If pure absorption occurs, the fraction of light absorbed depends both on the optical path length through the matter and on the physical-chemical characteristics of the substance according to the Lambert-Beer Law: -log I/Io = c d or A = c d Where: -log I/Io Io I c d = = = = = = Absorbance (A) intensity of incident light beam intensity of light beam after absorption molar extinction coefficient at wavelength molar concentration of the substance optical path through the substance · After a few seconds the display will show "-0. 0-". The meter is now zeroed and ready for measurement. Remove the Blank Vial. · Insert the Sample Vial into the instrument. Note: If the sample vial appears brown/orange without blue hue, dilute the wine sample and repeat the procedure. · Invert the vials two times to mix. Then wait for 30 minutes to allow the vials cool to room temperature. Note: This operation is necessary to recover the condensed water after heating. · Turn the instrument ON by pressing ON/OFF. [. . . ] 12 Batteries voltage is getting low and the batteries need to be replaced. 9 Indicates that the batteries are dead and must be replaced. After this message appears, the instrument is switched off. Change the batteries and restart the meter. [. . . ]