User manual HANNA INSTRUMENTS HI 3897

[. . . ] Instruction Manual Significance and Use Acidity defined as oleic acid, is a parameter that indicates the oil freshness: a high acidity value shows the oil is becoming rancid, thus the oil quality is decreasing. According to CEE 2568/91 regulation, olive oil is called extra virgin when its acidity is below 1%: see table 1. [. . . ] Note: Any damaged or defective item must be returned in its original packing materials. · Using the 1. 0 ml syringe with tip, add the HI 3897-0 Titrant Solution reagent dropwise. Continue adding the Titratant Solution reagent until the solution in the bottle changes from colorless to a persistent light pink. Note: Note stop adding the HI 3897-0 Titrant Solution reagent to the bottle as soon as the solution is persistently light pink colored. · Remove the cap and add the oil sample to the Organic Solvent bottle. Replace the cap. · Place the sample on the magnetic stirrer and stir gently until all the oil sample is dissolved in the Organic Solvent reagent. · Using the 1. 0 ml graduated syringe with tip, take 1. 0 ml of HI 3897-0 Titrant Solution reagent. Note: Note to dose exactly 1. 0 ml of the Titrant Solution reagent: (a) push the plunger completely into the syringe, (b) insert tip into HI 3897-0 Titrant Solution reagent, (c) pull the plunger out until the lower edge of the plunger seal is on the 0 mL mark of the syringe, (d) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are hanging on the tip of the syringe, if so eliminate them. Chemical Reaction The oil sample is firstly dissolved in an organic solvent reagent (ethanol/ether); then it is titrated with a basic solution (OH-). The final point of the titration is indicated by the change in color of the solution, which turns from yellow/green of the oil to pink. Specifications Range Smallest Increment Analysis Method Sample Size Number of Tests Case Dimensions Shipping Weight 0 to 1% acidity 0. 01 ml = 0. 01 % Titration 4. 6 mL (or 4. 0 g) 6 112 x 390 x 318 mm 3000 g Instructions READ ALL INSTRUCTIONS BEFORE USING THE TEST KIT CAUTION!Carefully open the bottle of the Organic Solvent reagent because it may be under pressure! 07/04 ISTR3897R2 · Take 4. 6 ml of oil sample by use of the 5. 0 ml syringe. Alternatively, weight about 4. 0 g ("w" grams) of oil by use of an analytical balance. Note: Note to dose exactly 4. 6 ml of the oil sample: (a) push the plunger completely into the syringe, (b) insert the tip into the oil sample, (c) pull the plunger up until the lower edge of the seal is exactly on the 5 mL mark, (d) take out the syringe and clean the outside of the syringe tip. Be sure that no drops are hanging on the tip of the syringe, if so eliminate them, (e) then, keeping the syringe in vertical position above the oil sample, push the plunger down into the syringe, till the 4. 6 ml sign, (e) be sure that no drops are hanging on the tip of the syringe, if so eliminate them, (g) then, keeping the syringe in vertical position above the (open) bottle of Organic Solvent, push the plunger completely down into the syringe. Now the exact amount of 4. 6 mL has been added to the bottle. · Remove the cap. · Hold the syringe in vertical position above the bottle. [. . . ] · To perform a new test, proceed as described in the "Instructions". Health and Safety data Sheets The chemicals contained in this test kit may be hazardous if improperly handled. Read Health and Safety Data Sheets before performing the test. ml of titrant = % oil acidity [i. e. : 0. 5 ml = 0. 5% oil acidity] - If "w" grams of oil sample was weighted and added to the bottle, then the acidity is : % oil acidity [. . . ]