User manual LEICA APPLICATION SUITE IMAGE OVERLAY BROCHURE

[. . . ] Leica Application Suite Image Overlay Simply Brilliant! Automatic image acquisition Precise control of microscope functions is essential for high quality fluorescence imaging. By displaying a live image with exposure adapted to the selected filter, it is easy to compose the image in the field of view. [. . . ] Other benefits include: · The image display adapts automatically to the resolution of the acquired or imported image including the ultra high resolutions provided by Leica DFC digital cameras. · Zoom and pan allows you to inspect an image area in detail. · The overlay image is saved in to a named folder, containing all channel and composite images as well as configuration details. Advanced Applications in Digital Fluorescence Microscopy Image enhancement The Leica Application Suite Image Overlay module enables images to be enhanced by applying a range of image processing techniques. This includes image contrast, brightness and gamma which can be adjusted to optimise the image display. Other benefits include: · Cameras can provide 8-bit or 16-bit images, in monochrome or RGB retaining the full dynamic range of the high bit-depth. · Exposure and gain can be set individually for each filter position. · Image mixing ­ images from fluorescence and other contrast methods can be collected without moving the slide and this allows the fluorescent information to be overlaid with for example, DIC to indicate regions of co-localisation. Annotation Once an image is enhanced satisfactorily, annotations can be applied to further high light areas of interest. · Images can be annotated with calibration markers for an obvious guide to size of microstructure. · Further annotations can be added such as the image name, date of acquisition and description. · Annotations can be merged into an image for a permanent record. LAS is based on Windows PCs and provides a cost-effective and uniform environment, compatible across the Leica range of microscopes and cameras. Furthermore, images may be exported for additional processing. Monitor Image: DIC & fluorescent overlay acquired with a Leica DM6000 B microscope LAS Image Overlay The Leica Application Suite Image Overlay module is a highly optimised, yet cost-effective imaging application, combining Leica's strengths in automated fluorescence microscopy, digital camera technology and advanced imaging. Constructed for the visualisation, enhancement and digital documentation of coloured fluorescence microscope images, it is extremely versatile, making it suitable for users in bioscience, medical and pharmacological laboratories. [. . . ] Printed on chlorine-free paper with a high content of recycled fibre. M1-525-1en · © Leica Microsystems (Switzerland) Ltd · CH-9435 Heerbrugg, 2005· Printed in Switzerland ­ VIII. 2005 ­ RDV [. . . ]