User manual LEICA APPLICATION SUITE MULTISTEP BROCHURE

[. . . ] Leica Application Suite MultiStep MultiStep Imaging! Automatic Image Acquisition LAS MultiStep is an optional module that when used with a Leica microscope, motorised scanning stage and digital camera, automatically acquires images at XY positions defined by a rectangular pattern. The camera can acquire images as soon as the stage has reached the specified position and halted. [. . . ] · Scanning sequences and microscope settings can be saved and recalled to increase efficiency whilst saving valuable time. Multiple Scanning! Scanning Definitions LAS MultiStep provides users with the opportunity to define scanning patterns specific to individual needs. This includes the ability to enter the dimensions of a specimen, establish the origin of the scanning pattern and set the magnification of the image, which in turn determines the number of fields acquired. Furthermore, the scanning pattern can be paused if the focal position needs adjusting or extending. The entire sequence can also be stored and recalled for any future routine requirements to save valuable time and increase efficiency by using stage scanning and focusing algorithms. Scanning Analysis During scanning analysis, LAS MultiStep enables users to correct the focus position through the «predictive focus» functionality. Having manually focused a number of defined areas of a specimen, a «best fit of focus plane» can then be calculated and used during subsequent analysis. Additionally, LAS MultiStep offers a reduction factor option so that large Mosaic images which are difficult to manipulate, can be reduced in size into more manageable dimensions. LAS is based on Windows PCs and provides a cost-effective and uniform environment, compatible across the Leica range of microscopes and Digital FireWire cameras. LAS MultiStep LAS MultiStep is a highly versatile imaging module that can be used with many specimen types for users interested in materials science, quality control, forensics, bioscience, pharmacology and many more areas. LAS MultiStep imaging can be used for applications such as: · Specimen Scanning ­ specify a repetitive rectangular scan pattern for automatic analysis defined by the number of X and Y steps and step sizes in each direction. · Mosaic Scanning ­ take images from a stage scan pattern and stitch them together to form an overview image. [. . . ] Printed on chlorine-free paper with a high content of recycled fibre. M1-525-5en · © Leica Microsystems (Switzerland) Ltd · CH-9435 Heerbrugg, 2005 · Printed in Switzerland ­ II. 2006 ­ RDV Order number 12730074 Leica Application Suite ­ MultiStep Module www. leica-microsystems. com/LAS [. . . ]