User manual LEICA CM1850

[. . . ] Cryostat Instruction Manual Leica CM1850 V2. 5 English ­ 04/2010 Always keep this manual near the instrument!Read carefully prior to operating the instrument! INFORMATION The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. For erroneous statements, drawings, technical illustrations etc. [. . . ] · Insert the shaft of the specimen disc (3) in the location hole (2) of the specimen head. Make sure that the shaft of the specimen disc is fully inserted. The entire rear surface of the prism must have a good contact with the specimen head. Fig. 18 · Retighten screw (1). 7. 2. 2 Specimen orientation · To release, loosen screw (4). · Orient the specimen surface with lever (5). · Retighten screw (4). Leica CM 1850 ­ Cryostat 27 7. 7. 3 Daily use of the instrument Sectioning All necessary adjustments on the knife holder and anti-roll guide are described in the separate instruction manual for your knife holder in detail. 7. 3. 1 Trimming the specimen Take care when handling microtome knives and disposable blades. The cutting edge is extremely sharp and can cause severe injury! · Insert the precooled knife/blade in the knife holder and clamp. · Adjust the appropriate clearance angle on the knife holder. Adjustments between 4 ° and 6 ° (knife holder CN and CE-TC) or between 1 ° and 2 ° (knife holder CE) are suitable for most applications. · Align the knife holder/knife with the specimen. · Remove the knife guard (knife holder CN) or fold the glass anti-roll guide (knife holder CE and CETC) over to the left. · Trim the specimen to shape approach the specimen towards the knife by means of the coarse feed buttons. Trim the specimen down to the required sectioning plane by turning the handwheel. · Position the anti-roll guide on the knife and align with the cutting edge. Readjust the anti-roll plate if necessary. 7. 3. 2 Section thickness setting The section thickness is adjusted in a range of 1 to 60 µm by turning the knob (1) : from 0 - 10 µm in 1 µm increments, from 10 - 20 µm in 2 µm increments, from 20 - 60 µm in 5 µm increments. The selected section thickness is indicated on the index mark on the microtome. · Select the required section thickness with the control knob on the microtome. Start sectioning at approximately 20 µm. · Decrease the section thickness continually down to the appropriate value. After changing from one section thickness to another, the first two or three sections should be rejected. · When sectioning, turn the handwheel at a constant speed. 28 Instruction Manual V 2. 5 ­ 04/2010 7. Daily use of the instrument 7. 4 Temperaturew selection chart (in minus °C) -10°C ­ -15°C -15°C ­ -25°C -25°C ­ -35°C Tissue Adrenal Bone marrow Brain Bladder Breast - fatty Breast - little fat Cartilage Cervical Fatty Heart and vascular Intestinal Kidney Laryngeal Lip Liver Lung Lymphoid Muscular Nose Pancreatic Prostate Ovarian Rectal Skin with fat Skin without fat Spleenal or bloody tissue Testicular Thyroid Tongue Uterus curettage The temperature values given above are based on long-term experience, however, these are only approximate values, as any tissue may require particular adjustments. Leica CM1850 ­ Cryostat 29 7. 7. 5 Daily use of the instrument Defrosting Defrosting the cryochamber actually means defrosting the evaporator to prevent excessive frost buildup. [. . . ] Insert the shaft (6) of the specimen disc (5) in the appropriate location hole (7) at the top or bottom of the thermal block. After about 20 seconds, the frozen specimen can be removed from the specimen disc with forceps. If the cap is too loose, readjust it with the small screw (10). Once the specimen is removed, take the thermal block out of the cold cryochamber. · · · 9 Fig. [. . . ]