User manual LEICA CM3050 S

[. . . ] Cryostat Instruction Manual Leica CM3050 S ­ Cryostat English V1. 5 ­ 03/2010 Always keep this manual near the instrument. Read carefully prior to operating the instrument. Important note The information, numerical data, notes and value judgments contained in this manual represent the current state of scientific knowledge and stateof-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. For erroneous statements, drawings, technical illustrations, etc. [. . . ] Preset counter CT-30°C OT - 35°C dn056 Meaning of the refrigeration symbols (asterisks): --> see chapter 5. 3. 5 `Status indication'. Section thickness CT-30°C OT - 35°C µ010. 0 or The sectioning thickness is set with the key (see chapter 5. 4. 7 `Setting the section thickness'). Section counter CT-30°C OT - 35°C n000 Setting preset counter to `0': key. Press can also be pressed while the continuous sectioning mode is in motion; in this case the preset counter is set to `0' and sectioning stops upon reaching the lower limit position. After it has been set to `0', the preset counter is inactive. Reloading the former reference value: Press and simultaneously. Reloading the former reference value is possible also while the continuous sectioning mode is in motion: Upon pressing both arrow keys, the instrument continues sectioning without interruption, starting to count down from the old reference value. Once displayed (via arrow keys), the section counter can be set to `0' at any time. · To set to `0' press If the section counter is not set to `0' by the user at some point, sections are counted up to `999' at which point the counter automatically switches back to `0'. Leica CM3050 S ­ Cryostat 33 5. 5. 4 Operation Control panel 2 Instruments with sectioning motor In instruments with sectioning motor, the control panel is equipped with three functional areas: A, B and C (see large diagram). Instruments without sectioning motor In instruments without sectioning motor, control panel 2 only consists of functional area B (see below) A Functional area A: · Sectioning modes · Sectioning window · Sectioning speed · Emergency stop function B Functional area B: · Coarse feed · Trimming · Section thickness selection B Functional area B: · Coarse feed · Trimming · Section thickness selection C Functional area C: · Start / Stop sectioning 34 Instruction Manual V1. 5 - 03/2010 5. Operation Functional areas A and C - Instruments with sectioning motor only 5. 4. 1 Selecting a sectioning mode / start and stop sectioning Three different sectioning modes can be selected for motorized sectioning: Sectioning mode 1 - Intermittent stroke: The specimen head travels up and down as long as the RUN/STOP and RUN/ENABLE keys are pressed simultaneously or as long as the footswitch is depressed. Sectioning mode 2 - Continuous stroke: The specimen head travels up and down until it is stopped by pressing RUN/STOP or RUN/ENABLE or by depressing the footswitch. Sectioning mode 3 - Single stroke: The specimen head carries out one sectioning stroke and then stops in the lower limit position. Activating a sectioning mode Press one of the three sectioning mode keys described above. While a sectioning mode is active, the LED in the corresponding key will be illuminated. Start / stop motorized sectioning Sectioning mode 1 - Intermittent stroke: · Simultaneously press and hold RUN/STOP and RUN/ENABLE or depress and hold footswitch. · To stop the sectioning stroke, release the keys or the footswitch: The specimen head stops immediately. Sectioning mode 2 - Continuous stroke: · Simultaneously press and release RUN/STOP and RUN/ENABLE or depress and release footswitch. · To stop sectioning: Press and release RUN/STOP or RUN/ENABLE or depress and release the footswitch: The specimen head stops upon reaching the lower limit position. Sectioning mode 3 - Single stroke: · Simultaneously press and release RUN/STOP and RUN/ENABLE or depress and release footswitch. · The instrument carries out one complete section / retraction stroke and the specimen head stops upon reaching the lower limit position. While sectioning is in motion, LED `RUN' is illuminated. While the sectioning motor is deactivated, LED `STOP' is illuminated. Both LEDs are located in functional area C. Leica CM3050 S ­ Cryostat 35 5. Operation Functional areas A and C - Instruments with sectioning motor only 5. 4. 2 Setting the sectioning window The term `Sectioning window' stands for a different (slower) speed setting in that part of the sectioning stroke where the specimen is actually cut, while the remaining sectioning stroke (above and below the specimen) as well as the retraction (return) stroke are carried out at a higher speed level (time-saving feature). If a sectioning window is set, speed inside the window is as set on the control panel. [. . . ] 14 0712 40151 Easy Dip staining cnt. grn. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . yl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . bl . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . rack grey . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ]