User manual LEICA CRYOSTATS DISINFECTION PROTOCOL

[. . . ] Disinfection Protocol for Cryostats Universal Precautions: Treat ALL human blood, tissue, body fluids, and matter as if you know for certain that they are infectious. Definition of Disinfection: Disinfection is the destruction of pathogens by physical or chemical means. [. . . ] Trimmings and any solid waste material MUST be removed from the instrument prior to disinfection. Spray exposed surfaces with 70% alcohol or Cryofect disinfectant (Cryofect cannot be sold in the USA. ). Make certain that the sliding window is properly closed and select the appropriate exposure time. The CM1850 UV provides a safe and convenient method for disinfection of surfaces and air within the irradiated working space at cold temperatures and significantly reduces the risk of infection. Intermediate-level disinfection can be accomplished using short-term irradiation (30 minute cycle). High-level disinfection can be accomplished using long-term irradiation (180 minute cycle). Please note that ALL visible contamination must be removed from the chamber before using the UV C light and that the germicidal effect of the radiation is restricted to directly illuminated areas. Exposure to UV irradiation cannot replace chemical disinfection. For more extensive disinfection, bring the cryostat to room temperature and wipe the exposed surfaces with an EPA approved tuberculocidal disinfectant. The EPA publishes a list of approved disinfectants. Follow the directions written on the containers. Allow at least three hours to take the cryostat down to operating temperatures. Wear double gloves, gown, mask, and protective eyewear. Be certain to remove any disposable blades/knives before beginning and disinfect them separately. [. . . ] Let the solution sit for 10 minutes, drain, then wipe with absorbent towels. Drain, and allow to air dry or wipe with absorbent towels. [. . . ]