User manual LEICA CV ULTRA DATASHEET

[. . . ] Replacement of Xylene by Leica ST Ultra and Leica CV Ultra Leica ST Ultra and Leica CV Ultra have been developed to replace Xylene/Toluene and conventional mounting media in histopathology/Cytopathology laboratories. Leica ST Ultra is used for clearing; Leica CV Ultra is used for coverslipping (mouting) Leica Microsystems Online Product News Leica ST Ultra Leica CV Ultra Leica ST Ultra and Leica CV Ultra both are: Non flammable Odourless No detectable aromatics Non carcinogenic Non poisonous Environmentally and human friendly There are two typical processes in a histo/cytopathology laboratory: - Tissue Processing - Deparaffination, Staining and Coverslipping Change to Leica ST Ultra in Tissue Processing The general procedure for tissue processing is documented below, although slight variations exist from one laboratory to another. Sequence Formaldehyde 10 % v/v Formaldehyde 10 % v/v Ethanol 50 % Ethanol 70 % Ethanol 100 % Ethanol 100 % Ethanol 100 % Ethanol 100 %* Xylene Xylene Paraffin Paraffin Paraffin Paraffin Time in minutes 30 60 30 30 30 60 60 120 40 80 ** 60 60 60 120 Temperature °C 40 40 40 40 40 40 40 40 40 40 60 60 60 60 Leica Microsystems Online Product News Leica ST Ultra Leica CV Ultra If Xylene is replaced by Leica ST Ultra it is important to note the following: * More frequent refreshment of 100% Ethanol for tissue dehydration might be necessary since xylene is partially miscible with water. [. . . ] Tissue slices too thick Tissue not completely deparaffinated. Slide appearance is milky during coverslipping Leica ST Ultra use in final bath, together with a conventional mounting medium. Refresh final bath of 100 % Ethanol more frequently. De-paraffination problems Leica ST Ultra saturated with paraffin. Last 100 % Ethanol bath in dehydration process should be refreshed. Leica CV Ultra versus Pertex Cytology lab technicians sometimes prefer Pertex, because of very quick fixing. Tests were done and Pertex mounts a bit faster than CV Ultra. Initial fixing takes about 5 min versus 10 min for CV Ultra. But after 1 hour the CV Ultra and Pertex mount the coverglass to the same extend. Details Leica Microsystems Online Product News Leica ST Ultra Leica CV Ultra Sticking performance tests were done, by mounting a coverglass on a microscopic slide after a bath of Xylene. Xylene was used, because Pertex infridges with Leica ST Ultra. * The time needed for the initial fixing was checked. Also viscosity has been checked and Pertex and CV Ultra have more or less the same viscosity. So there is a small difference but for cytology use CV Ultra is very well applicable. Please find an overview of the properties of both products in the table below. * Leica ST Ultra will be available from Dec. 2001 on. Criteria Initial mounting Mounting after one hour Contains Xylene Viscosity Can be used with Xylene, STUltra, IPA Thickens when used Leica CV Ultra 10 min. [. . . ] This reduces solvent usage substantially Leica ST Ultra and Leica CV Ultra are tradenames of Leica Microsystems. Leica ST Ultra and CV Ultra are manufactured by J. T. Baker, Netherlands for Leica Microsystems. M I C R OSYST E M S Leica Microsystems Nussloch GmbH, Postfach 1120, Heidelberger Strasse 17-19, D-69226 Nussloch, Germany Phone: +49-6224-143-0, Fax: +49-6224-143-105 [. . . ]