User manual LEICA CV5030

[. . . ] Leica CV5030 Robotic Coverslipper Operating Manual Leica CV5030 V2. 1 English - 08/2008 Always keep this manual near the instrument. Read it carefully prior to operating the instrument! NOTE The information, numerical data, notes and value judgments contained in this documentation represent the current state of scientific knowledge and state-of-the-art technology as we understand it following thorough investigation in this field. We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical developments, nor to provide our customers with additional copies, updates etc. For erroneous statements, drawings, technical illustrations etc. [. . . ] Cologne Medite BDH Laboratory Supplies England, Gurr O. Freiburg Surgipath 10 8 6 8 4 8 10 10 10 10 8 10 10 10 4 8 1-2 3 2 2 1 2 2 3 2 3 1 5 6 6 1 1 20 18 18 18 21 18 20 20 18 18 20 18 20 20 21 18 LEN: -40 STP: +40 LEN: -50 LEN: -50 LEN: -100 STP: -50 LEN: -50 LEN: -50 LEN: -50 STP: +50 LEN: -50 LEN: -50 LEN: -50 LEN: -50 Eukitt Micromount Protocol Xylene coverslipping mountant Fisher Health Care Poly Mount Poly Scientific ST Ultra-based: CV Ultra Leica 10 3 18 38 Operating Manual V 2. 1 ­ 08/2008 5. Operation Brand name Manufacturer Mountant Mountant type volume Nozzle size Correction values Toluene-based: Coverslipping mountant toluene Acrylmount toluene Acrytol coverslipping mountant toluene Cytoseal 280 Coverslipping mountant toluene Coverslipping mountant T Toluene Richard Allan Scientific Anapath Stat Lab Surgipath Stephens Scientific 10 8 8 10 2 4 7 8 21 21 21 21 LEN: -50 LEN: -50 Acra Lab Protocol Secure Mount Fisher Health Care coverslipping mountant toluene Protocol Fisher Scientific Company coverslipping mountant toluene 4 or 4 5 2 1 3 21 21 20 LEN: -100 LEN: -50 LEN: -50 STP: -50 LEN: -50 1 1 21 Not recommended: Neo-Mount: (tested with Neo-Clear/Merck) Roti-R-Histokitt (tested with Rotihistol/Roth) Reason: These coverslipping mountants have a very low viscosity that is not suitable for coverslipping machines. Therefore, the mountant is not applied uniformly, and excess mountant escapes from the cover slip. Optimized volume settings for other coverslipping conditions depend on and are affected by a variety of factors: Coverslipping condition Dry coverslipping High temperature (> 28 °C) Low temperature (< 2 °C) Effect on the volume setting more less more Coverslipping condition Cytology Single-layer cytology Cover slip size 40 mm Cover slip size 60 mm Effect on the volume setting more less less or shorter stroke length much more or longer stroke length Leica CV5030 ­ Robotic Coverslipper 39 5. Operation 5. 5 Displays ­ status messages The display of the Leica CV5030 is used to display status and error messages that provide information about the current status of the instrument. Status messages always appear in the bottom line of the display. The top line displays the operating status of the instrument. Therefore, the actual display may in some instances differ from that shown in the table. The following table compiles all status messages. In addition, it indicates to the user which action to carry out. All error messages displayed on the control unit of the device are listed in Chapter 7, "Malfunctions" (p. 61). Display Cause Appears after the instrument is switched on. Indicates that the instrument is being initialized. The user is prompted to check the loading bath. Action Do not press any button; wait until the next message appears. Open the door towards the front and pull out the bath; re-insert the bath and close the door. Move the dispenser into the prime position (park position). Press the PRIME button. The bath drawer is open. The user is prompted to move the dispenser into prime position (park position). The user is prompted to press the PRIME button in order to create the working pressure in the dispenser system. The working pressure is built up in the dispenser system. Wait until the working pressure is reached. You can continue working by pressing START. 40 Operating Manual V 2. 1 ­ 08/2008 5. Operation Status messages (continued) Display Cause The user is prompted to press the PRIME button in order to fill the dispenser with mountant. The user is prompted to move the dispenser into working position. The initialization is complete and the instrument is ready to operate. The user has pressed the PAUSE button and the current coverslipping operation is completed. [. . . ] 68 Specimen slide holder 30, Plastic, pack of 5 Order No. 69 Leica CV5030 ­ Robotic Coverslipper 71 8. Optional Accessories Specimen slide holder 30, Modified, plastic, 1 piece, for Varistain adapter (14 0464 37659) Order No. 70 Varistain adapter, Adapter for Shandon Varistain 24-4, Note: For use with Leica specimen slide holder 30, modified, plastic 14 0478 38029, to combine Varistain 24-4 with CV5030. [. . . ]