User manual LEICA DFC360 FX DATA SHEET

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Manual abstract: user guide LEICA DFC360 FXDATA SHEET

Detailed instructions for use are in the User's Guide.

[. . . ] Leica DFC360 FX High Speed Camera System for Fluorescence Microscopy Ultrafast High-Resolution Fluore Leica DFC360 FX ­ High-End Live Cell Imaging Speed is often the deciding factor for successful imaging in demanding high-resolution image documentation of living cells, molecular processes and rapidly fading fluorescence specimens. The monochrome digital camera Leica DFC360 FX is designed to produce brilliant images at maximum temporal resolution. [. . . ] The Leica DFC360 FX is the ideal solution for excellent results in live cell imaging. 2 scence Image Recording Leica Imaging ­ Ultrafast Image Recording Extremely high frame rates determined only by the necessary shutter speed: With the overlapping mode, the 2/3" Progressive Scan CCD sensor reads out imaging data while the next exposure is being taken. The optimized sensitivity of the sensor allows shorter exposure times for rapidly fading fluorescence specimens and a significant reduction of the excitation light for sensitive living cells. Microlenses on each chip ensure a high quantum yield right into the near infrared range. The light sensitivity and the frame rate can be further enhanced by binning for example over 100 fps with 8x8 binning. If the picture area is reduced for detailed analysis, the recording speed increases. Binning and region of interest can be used, as can multi-channel experiments with different amplifications, in the fast overlapping mode. The active Peltier cooling minimizes the dark current and guarantees noise-free images even at low light intensities. With shutter speeds of 4 µs to 10 minutes and up to tenfold signal amplification, the Leica DFC360 FX offers maximum flexibility. Convenient, Intuitive Operation The Leica Application Suite LAS enables intuitive, convenient image acquisition and image processing. Leica's LAS AF Advanced Fluorescence Software is ideal for swift and effective control of experiments such as z-stack, multi-channel fluorescence with overlay, 3D deconvolution, timecontrolled analysis and many other applications. [. . . ] of the editions in: English 914 636 · German 914 635 · French 914 637 · Spanish 914 638 · Italian 914 639 · Part-No. LEICA and the Leica Logo are registered trademarks of Leica IR GmbH. FireWire IEEE1394b [Frames/s] 20 39 58 71 109 [. . . ]

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