User manual LEICA EM HPM100 APPLICATION NOTE

[. . . ] Leica EM HPM100, Leica EM VCT100 High Pressure Freezing, Cryo-SEM Superior Cryopreparation of Biological and Industrial Samples Commercially available hand creams Application Note Leica EM HPM100, Leica EM VCT100 Commercially available hand creams Protocol: Hand creams having different water contents were applied into the 100 µm cavities of two 3 mm type A sample carriers which were then closed cream sides inwards. [. . . ] Samples were fractured by pushing the top carrier off with the fracturing knife at -140°C, then they were high angle rotary shadowed with platinum/carbon. The samples were again withdrawn into the EM VCT100 shuttle and then moved to the cryo-SEM. They were imaged with a Hitachi S4700 FE-SEM with the EM VCT100 CryoStage at ­125°C. Summary: Procedure High pressure freezing Freeze fracture Coating Transfer Imaging Results: High pressure freezing followed by freeze fracture and cryo-SEM are well-suited for determining the structure of water-containing industrial materials. Multiple samples can be processed and examined within a short time with an SEM equipped with a Leica EM VCT100 cryo-stage. [. . . ] at the University of British Columbia, BioImaging Facility, Vancouver, Canada [. . . ]