User manual LEICA EM RTS BROCHURE

[. . . ] Safety and reproducibility for the specimen are increased while operator mistakes are reduced. Structural details of the C. Müller-Reichert (MPI-CBG, Dresden, Germany) and Kent McDonald (University of California, Berkeley, USA) 2 Leica Design by W. Hölbl 3 Leica EM PACT2 ­ High Pressure Freezer Perfect results ­ High cooling rates by strong jet of LN2 ­ 120 samples can be frozen per hour ­ Variety of specimen carriers available for all purposes Easy to install ­ Compact and mobile ­ Standard electrical supply ­ Compressor on EM PACT2 trolley Easy to use ­ Touch-sensitive color screen, menu prompts ­ Bayonet loading device for automatic orientation ­ Specimen ejection into LN2 bath ­ LED bath illumination ­ 600 ms temperature/pressure curve displayed for each run ­ Internal memory for 8 000 freezing runs ­ Data download on memory stick Safe and convenient ­ Low LN2 consumption ­ Dewar with drain outlet ­ Universal hydraulic system ­ can even use water ­ Low noise ­ Maintenance free "long life pods" Function: All You Need is LN2. . . [. . . ] Directly suck the suspension into copper tubes (inner ø 350 µm) already mounted in their holder. Alternatively, take up sample into cellulose microcapillary. With the loading device the specimen can be inserted into the EM PACT. After cryofixation the samples are stored under LN2 in the transfer box. Sample Punch: punching out of the central part of the specimen tube ready for Frozen Hydrated Sectioning or . . . . . . peeling away of the top of the copper tube for Freeze Substitution. Storage of the copper tubes is carried out under LN2. The specimen tube holders are recycled by reloading them with copper tubes. Leica Design by W. Hölbl 7 Leica EM RTS Application Solutions Taking microbiopsies for EM is now faster than ever before!Prepare the Microbiopsy Transfer Station for RTS under the Optical Workstation. . . before taking a biopsy with the biopsy gun. Insert the biopsy gun into the transfer station . . . . . . and transfer the tissue into the biopsy carrier. The specimen and carrier are held in the rapid loader . . . . . . while the primed loading device waits for freezing in the RTS. By gently pushing the rapid loader into the RTS the carrier is tightened securely and then frozen automatically in less than 2. 5 seconds. After cryofixation the carriers are collected in the LN2 bath of the EM PACT2 before freeze substitution in the Leica EM AFS2 or cryosectioning in the EM FC6. 8 Frozen Hydrated Sectioning made easy. Alternatively, the carriers can be trimmed with a cutter . . . . . . for Frozen Hydrated Sectioning in the cryoultramicrotome. All necessary steps for follow-on procedures (eg. Freeze Substitution) can be conveniently performed in the cryopreparation box supplied with the Leica EM PACT2. The Freeze Fracture Holder for everything that can be fractured . . . Prepare the Freeze Fracture Station under the Optical Workstation . . . and preload the freeze fracture carrier. [. . . ] Copyright © · Specimen Preparation We provide comprehensive systems and services for clinical histo- and cytopathology applications, biomedical research and industrial quality assurance. Our product range includes instruments, systems and consumables for tissue infiltration and embedding, microtomes and cryostats as well as automated stainers and coverslippers. · Medical Equipment Innovative technologies in our surgical microscopes offer new therapeutic approaches in microsurgery. and representatives of Leica Microsystems in more than 100 countries. · Semiconductor Equipment Our automated, leading-edge measurement and inspection systems and our E-beam lithography systems make us the first choice supplier for semiconductor manufacturers all over the world. @ www. em-preparation. com Leica Mikrosysteme GmbH Hernalser Hauptstrasse 219 A-1170 Vienna Austria Tel. +43 1 48899-235 Fax +43 1 48899-350 Leica EM PACT2_EM RTS - E - 6/05 Order No 206002, Printed on chlorine-free bleached paper. [. . . ]