User manual LEICA HCS A BROCHURE

[. . . ] Leica HCS A Amplify the Power of Imaging High Content Screening Automation 1 2 · Amplify the power of confocal imaging with Leica HCS A · Maximum flexibility for universal applications · Easy-to-use automation provides efficient high content screening · Powerful hardware for high resolution imaging and maximum content 2 Leica Design by Christophe Apothéloz High Content Screening (HCS) allows researchers to quickly change from descriptive to quantitative fluorescence imaging during an experiment. High resolution imaging automation therefore answers complex questions in shorter time. It simplifies research work and efficiently reveals relationships within and between cells and organisms. Leica Microsystems offers a set of innovative tools to convert your high resolution confocal microscope into a high content imaging device. Leica HCS A High Content Screening Automation Leica Microsystems provides a wide range of true spectral confocal imaging systems, known for brilliant image quality and maximum resolution. [. . . ] Review On-the-Fly Data is stored at predefined locations on a local hard disk or network storage device (NAS) via a TCP/IP protocol. Experiment data flows into a ring buffer to ensure an unlimited stream of images enters the specified target folder. The advantage: data analysis or review of image data is performed immediately. Image analysis starts when the experiment starts and provides fast results. Fast feedback loops automatically communicate with the microscope as it scans to automatically modify the scanning parameters. This enables researchers to detect rare events at the moment they happen. Autofocus Procedure Z-stack images are acquired at freely selectable positions. The focus positions are determined and stored in a color coded focus map. 11 Automated High Content Screening for Quant Detect Rare Events On-the-Fly! LAS AF MATRIX Developer Suite Highly complex assay designs are now possible with LAS AF MATRIX Developer Suite. The Mitocheck project (1), conducted at the EMBL in Heidelberg, is an excellent example of comprehensive and flexible automation: The self-acting process automates mitosis identification. Pre-Scan ­ Object Identification At first, a fast, low resolution pre-scan of sample plates is routinely performed to identify the events of interest (1). Experiment Meta Data Administration Experiment IDs, description, and meta data can be entered manually or by barcode. Additional experiment information can be added to the existing XML meta data file by external programming to provide comprehensive result data sets. Platform Independent Results Distribution Leica HCS A imaging formats can be used platform independent on Apple MACTM OS, Microsoft Windows® or LINUX1 platforms. The new Data Exporter provides OME-TIFF image files automatically, containing binary image data plus XML meta data structure. Image Analysis Leica HCS A export formats are easily imported into all modern image analysis solutions such as e. g. DEFINIENS Cellenger®, ImageJ2, or MetaMorph®. Ensuring full compatibility to modern analysis platforms provides new options for target recognition, analysis and decision-making. Researchers benefit from applying existing algorithms or may create new ones to analyze data automatically. Automated image analysis of multiple assay by DEFINIENS Cellenger®, Courtesy of Dr. Pepperkok, European Molecular Biology Laboratory (EMBL), Heidelberg, Germany. MACTM OS X Windows® Linux1 Modern analysis tools offer solutions to identify, count, and measure target cells to obtain statistically relevant results. In addition, DEFINIENS Cellenger® quantifies relationships between target objects even in 2D or 3D. End users are free to choose among local, server-based, or clustered node analysis methods to maximize throughput and efficiently achieve high content screening results. 14 Transparency of High Content Data Data Model In the past, the original imaging and meta data had to migrate through a myriad of different conversion formats before ending up in a condensed Excel or Word document. Loss of data due to conversion is a problem of the past. The Leica export format follows the conventions of well-defined and well-formed structures, and can be read by all modern software platforms. Transformation problems, data mix-up or transcription errors are avoided and processing time is saved. [. . . ] A special glass prism provides the transmission for spectral analysis. 4 Drosophila, Leica TCS SP5 5 Fibroblasts, Leica TCS SPE 6 Mouse embryo, Leica TCS LSI Leica TCS SP5 18 Leica TCS SPE Gain Scientific Advantage Intelligent Automation The Leica HCS A technology provides an excellent platform for fast, efficient high content screening results. Leica combines highest resolution for all specimen sizes with LAS AF MATRIX M3 automation software. Maximum application flexibility for all automation levels creates unrivaled freedom for experiment designs of today and tomorrow. Standardized interfaces integrate Leica HCS A platforms with every laboratory environment in an optimal way. [. . . ]