User manual LEICA IN VITRO FERTILIZATION BROCHURE

[. . . ] The Leica Microsystems inverted microscopes are especially appreciated for their superior optical performance in imaging oocytes, sperms and injection capillary. Stability and robustness are matters of course to provide you with best tools for your work. Courtesy of: C. Mehnert, Zentrum für In vivo Fertilization, Giessen, Germany The Tokaihit MATS, heating plate becomes flush with the stage surface to ensure easy handling of the specimens and easy operation of the manipulator The perfect ICSI workstation: Leica DMI3000 B, 3-plate stage, condenser S40 for modulation contrast for objectives 5x to 63x, new Narishige manipulators with injection units, heating stage 37°C. 2 Zoom in on the Sperm The method of intracytoplasmic morphologically selected sperm injection (IMSI) has gained a lot of interest in the last years. [. . . ] A full range of ergonomic equipment like tilting tubes, height adjustable focus knobs, and ergonomical stages make work more comfortable, pleasant, and fatigue-free. MAKLER Counting Chamber for rapid sperm analysis perfectly fits the Leica DM1000-3000 stages for convenient evaluation. Ideal solution for sperm analysis: the unique ergonomic system microscopes Leica DM1000, DM2000, DM2500 and DM3000 with heating stages 37°C. 4 Decoronization and Zygote Observation After the collection of the oocytes via follicle puncture, the oocytes must be stripped of their granulosa cells. This delicate and important work requires excellent stereoscopic, 3D visualization combined with a long working distance to offer easy and convenient access for the tools of the operator. After fecundation, the zygotes are observed to check the number, size and location of the pronuclei. The stereomicroscope is one of the optical tools used for visualization of the development from the zygote to the blastocyst state. Highest image quality combined with ergonomic design, ease of use and reliability are crucial for this essential step within assisted reproduction. The new Leica stereomicroscopes are available in manifold configurations offering highest modularity and accessories. The new Leica FusionOpticsTM has again exceeded the limits in resolution and depth of field. The oocyte in stereo: · Brilliant image quality · Perfect stereo impression also with spectacles · High working distance up to 135 mm · Zoom up to 160x (1x objective) · Stable and vibration free design · Manifold accessories, heating stage, camera, software Human oocyte before decoronization Human oocyte after decoronization Highly effective instruments for manual inspection: Leica M125, M165 C, M205 C modular stereomicroscopes. C. Wittemer CMCO-SIHCUS Schiltigheim (France) 5 Advanced Analysis Tools for Cytogenetics Leica CW4000 CytoFISH at a glance · Acquisition facilities for up to 16 image planes, allowing the capture of standard FISH, MFISH and CGH images · Autoexposure facility can be used to provide a `one touch' fully automatic capture sequence · Capture a region of interest in the camera field of view, or capture using the entire CCD array for the highest spatial resolution. Binning can optionally be used to increase sensitivity · No need to threshold images to generate composite images ­ this removes the possibility of excluding faint `real' signals from the image · Full compatibility with karyotyping applications to allow flexible karyotyping of 2, 3 or multicolor images The Leica CW4000 CytoFISH provides fluorescence image acquisition and advanced analysis tool for prenatal diagnostics (PGD). It includes the ability to count FISH signals in interphase cells and to perform a ratio of the number of two different color FISH spots. The Leica CW4000 software is fully compatible with the Leica digital microscopes DM4000­6000 B and Leica FireWire cameras. The user can combine brightfield and fluorescence images to view CISH or FISH images and combine immunostaining with FISH experiments. Leica CW4000 CytoFISH offers a range of easy to use tools to adjust contrast, color and definition of objects in the acquired images. The modular system includes a full range of Karyotyping, FISH, CGH and MFISH options, providing outstanding accurate, detailed and high quality images and data. Different DNA areas on different chromosomes are marked using fluorescent probes. Courtesy of: Guangzhou No. 2 Ren Min Hospital ­ O&G Institute; X. [. . . ] +1 847 405 0123 Fax +61 2 9878 1055 Fax +43 1 486 80 50 30 Fax +32 2 790 98 68 Fax +1 905 762 8937 Fax +45 4454 0111 Fax +33 1 56 05 23 23 Fax +49 64 41 29 41 55 Fax +39 02 574 03392 Fax +81 3 5421 2896 Fax +82 2 514 65 48 Fax +31 70 4132 109 Fax +852 2564 4163 Fax +351 21 385 4668 Fax +65 6773 0628 Fax +34 93 494 95 32 Fax +46 8 625 45 10 Fax +41 71 726 34 44 Fax +44 1908 609 992 Fax +1 847 405 0164 Order no. : English 914 693 · VI/09/DX/BR. H. LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. · Industry Division The Leica Microsystems Industry Division's focus is to support customers' pursuit of the highest quality end result. Leica Microsystems provide the best and most innovative imaging systems to see, measure, and analyze the microstructures in routine and research industrial applications, materials science, quality control, forensic science investigation, and educational applications. · Biosystems Division The Leica Microsystems Biosystems Division brings histopathology labs and researchers the highest-quality, most comprehensive product range. [. . . ]