User manual LEICA LED5000 HDI

[. . . ] Using the Leica Application Suite software (LAS), userscancontrol, save, andlatercallup scenarios with different microscope and light settingsatthetouchofabutton. We wish you great joy and success with your newLEDilluminatorbyLeicaMicrosystems! Constant color temperature (daylight) throughouttheentirelifecycle Extremelylongservicelifeofupto50, 000 hours Absolutely maintenance-free; no lamp replacementrequired Leica LED Illuminator Manual 6 Control via LAS and Leica SmartTouch® Each Leica LED illuminator can also be controlled via the Leica Application Suite (LAS) or theLeicaSmartTouch®controlunit. can be assured that you will have consistent conditionsforyourexperiments. For information on how to control the LEDilluminatorviaLAS, refertotheuser manualfortheLASsoftware. Leica Application Suite (LAS) Leica SmartTouch® AllLeicaLEDilluminatorscanalsobeusedwith the Leica SmartTouch® control unit. [. . . ] With all other objectives, theworkingdistanceistoolowfor adequateillumination. The ring illuminator cannot be used together withtheobjectivenosepiece. · objectiveasfarasitwillgoandscrewit intoplace. 2. The flat angle of the oblique incident light creates a particularly high contrast for viewing the specimen, allowingtheusertodetectminuteunevenness and faults, e. g. In contrast to the goose-neck illuminator, the settings of the LED5000 MCITM are fully reproducible. Advantages Ninehigh-performanceLEDsfromdifferent anglesanddirections Highcontrastallowsuserstodiscoverfine structuresonthespecimen Illuminationangleof15­40° Reproducibleilluminationsettings Optimumaccesstospecimen Of course, the Leica LED5000 MCITM can also be controlled by the LAS (Leica Application Suite) software. Leica LED Illuminator Manual 29 Leica LED5000 MCI: Assembly The Leica LED5000 MCITM (for "Multi Contrast Illumination")isinstalledusingtwoscrews. For optimumaccessibility, theopticscarriershould beremovedduringinstallation. Hold the LED5000 MCITM with one hand and tightly screw the retraining stirrupsonbothtopholesonthedrivehousing. ConnecttheCAN-buscabletoeitherofthe twosockets. (Theflatpartoftheplugmust befacingdownwards. ) · 3. Plugtheotherendofthecableintooneof thetwo"CTL2"socketsonthecolumn. Constraints The Leica LED5000 MCITM cannot be used togetherwiththeobjectivenosepiece. Leica LED Illuminator Manual 30 Leica LED5000 MCI: Alternative Assembly Under certain circumstances, the light source must not be moved along with the optics carrier. Atypicalexampleismultifocusimages in which the Z-stack changes while the angle ofincidenceofthelightmustremainthesame. For such purposes, the Leica LED5000 MCITM is directlyfastenedtothecolumn. Pull the retaining stirrup out of the Leica LED5000MCITM. rup. If using the AX carrier, use the upper engaging position of the retaining stir- 3. Push the Leica LED5000 MCITM onto the retainingstirrup. 2. Screwtheretainingstirrupintothecolumn atthenotchusingthesinglescrew. Ifyou tighten the screw, the retaining stirrup is automaticallymovedintothecorrectposition. Leica LED Illuminator Manual 31 Leica LED5000 MCITM: Use Use The Leica LED5000 MCITM (for "Multi Contrast Illumination") is a universal high-output illuminator. Three groups of 3 LEDs each can be arrangedasdesiredaroundthespecimen. The targeted control of the LEDs enables various illuminationscenariostobeimplemented. Preparation HoldtheMCIwithbothhandsandpullitdownwardsuntiltheilluminatorclicksintoplaceon thebottomendoftheguiderods. Inthisposition, youalwayshavethesamecontrastwithidenticalillumination. Thisguarantees thereproducibilityofanexperiment. Optimum height Installed too high Contact with the base If the optics carriers are accidentally lowered too far, contact between the baseandMCImayresult. Asafetymechanism inthelinkageensuresthatinthiscase, theMCI isautomaticallypushedupwardstopreventthe possibilityofdamage. · Besurenottoplaceanyspecimensdirectly beneaththeMCI. Aftertheopticscarrierislifted, puttheMCI backintotheoriginalposition. Leica LED Illuminator Manual 32 Leica LED5000 MCITM: Use (cont'd. ) Using the keyboard Usethe keytoswitchtheilluminationon oroff. The illumination scenarios Usethe" "and" "keystotogglebetweenthe illuminationscenariosshownbelow. Maximum brightness For temperature reasons its is not possible to switchonallnineLEDssimultaneously. Therefore, toprovidebrightoverallillumination, the top two rows are activated. The bottom row primarilyprovidescontrast. Use the "+" and "­" keys to adjust the brightness in 10 increments. Touch either ofthetwobuttonstoadjusttheintensityin smallincrements. Holdakeytochangethe intensitymorequickly. The Leica LED5000 MCITM can also be controlled by the LAS (Leica Application Suite) software. Maximum brightness Maximum contrast Point illumination from the rear Point illumination from the left Point illumination from the right Leica LED Illuminator Manual 33 Leica LED5000 MCI and Leica SmartTouch® illumination scenarios for the Leica LED5000 MCI("MultiContrastIllumination"). control both the brightness and various Using the Leica SmartTouchTM, you can Adjusting the illumination rightsideofthedisplay. Theselectedbrightnessisshownonthe 5. Touch one of the symbols for the light scenarios to adjust the lighting to your needs. [. . . ] Thankstothehighflexibility, itisalsopossible tojustfoldupordownoneside. The illumination scenarios The flexible plastic dome is the central feature of the Leica LED5000 HDITM. It allowstwoilluminatoroperatingmodes. Use the button to switch between the three illumination modes: Both LED rings active>TopLEDringactive>BottomLED ringactive IfbothLEDringsareactive, onlythebrightnessofthetopringcanbechanged. Dependingonthespecimenandrequirements, it is thus possible to attain balanced brightnessconditionsforbothrings. You can switch between the two modes by moving the dome upwards or downwards at thetwotabsontheleftandrightofthemetal ring. Leica LED Illuminator Manual 46 Leica LED5000 HDITM: Illumination Scenarios (continued) The use of flexible plastic eliminates "hard collisions" with specimens to a large extent. For very sensitive specimens, however, even the weight of the illuminator itselfcancausedamage. 1. Dome folded down Inthismode, theLeicaLED5000HDITMisparticularly powerful. [. . . ]