User manual LEICA LMD6500 BROCHURE

[. . . ] No additional steps or complicated methods are necessary. Most importantly, the specimens are not touched. This makes specimen collection by gravity a contamination-free procedure. Transfer dissectates of any size or shape Gravity is the ideal tool to collect dissectates of any size or shape ­ round and compact, or long and thin ­ it simply falls into the collection device. [. . . ] This tool can also be used for real-time ablation of tissue. An important effect of laser beam movement is the ability to conveniently document the dissection with movies. The image remains fixed and only the laser beam moves ­ just the way our eyes are used to. Benefits: Highest possible precision Maximal speed Real-time laser cutting Convenient movie documentation Maintenance free Step 1: Define region of interest Step 2: Laser beam steered by optics along the cut line Step 3: Specimen collection by gravity * Patented EP 1276586 B1, US 7035004 B2, JP 3996773 B2, TW 486566 B 5 6 Leading-edge Laser Technology The laser is the core part of a laser microdissection system. The two key features of a laser are pulse energy and pulse frequency. The combination of these features within a LMD system was realized by Leica Microsystems for the first time. Adapt the laser to the specimen The Leica LMD7000 allows the control of laser pulse energy and frequency. Adjust the laser according to the specimen and make narrow, powerful, and fast cuts within a single system. Leica Microsystems' LMD systems provide the flexibility to align the UV offset with respect to the focus of the visible light for the most precise cutting performance, for thick or thin samples, and ablation. Control the thickness of the cutting line The aperture control enables the adjustment of the width of the cutting line. Combined with the high repetition rate, the narrowest cuts are possible. The laser aperture settings can be stored individually for each objective. LMD Laser Systems Leica LMD7000 120 J Single pulse to 5000 Hz Yes 349 nm Yes Leica LMD6500 50 J 80 Hz No 355 nm Yes Benefits: Control the pulse energy and frequency (for Leica LMD7000) Adjust the width of the cutting line Control the UV offset Maintenance free solid state laser Longlife, solid state laser Leica Microsystems uses diode-pumped, solid state lasers. These state-of-the-art lasers are maintenance free and reliable, ensuring long lifetimes. Choose the laser according to your needs: the Leica LMD6500 offers a pulse energy of 50 J and a fixed repetition rate, whereas the Leica LMD7000 provides a pulse energy of up to 120 J and the unique flexibility to adjust power and frequency. Please visit www. leica-microsystems. com/lmd for more images and movies, application letters, etc. Max. pulse energy Repetition rate Adjustable repetition rate Wavelength Laser aperture control Fig. 1: Brain, frozen section of GFAP-immunopositive astrocytes. Deller, MD, Institute of Clinical Neuroanatomy, J. W. 2: Primary cell culture from pancreatic adenocarcinoma (PDAC) stained with hematoxylin, objective 10x (before cutting, after cutting and inspection). Funel, Department of Oncology, University of Pisa, Italy Fig. Feldman, University of California, Berkeley, USA Fig. Courtesy of Axaron Bioscience AG, Heidelberg, Germany 7 System Integration Benefits: Fully automated DIC Motorized fluorescence axis with 5- or 8-position filter cube turret Fluorescence intensity manager (FIM) Motorized Excitation Manager and fast Internal Filter Wheel (IFW) Convenient Leica SmartTouch touchscreen for controlling the automated modules All components of the Leica LMD systems are fully integrated and act in concert, building a winning team greater than the sum of its components. [. . . ] We have developed five brand values to live up to this tradition: Pioneering, High-end Quality, Team Spirit, Dedication to Science, and Continuous Improvement. For us, living up to these values means: Living up to Life. Active worldwide Australia: Austria: Belgium: Canada: Denmark: France: Germany: Italy: Japan: Korea: Netherlands: People's Rep. of China: Portugal: Singapore Spain: Sweden: Switzerland: United Kingdom: USA: Barcelona Kista Heerbrugg Milton Keynes Bannockburn/lllinois North Ryde Vienna Groot Bijgaarden Richmond Hill/Ontario Herlev Nanterre Cedex Wetzlar Milan Tokyo Seoul Rijswijk Hong Kong Lisbon Tel. +1 847 405 0123 Fax +61 2 9878 1055 Fax +43 1 486 80 50 30 Fax +32 2 790 98 68 Fax +1 905 762 8937 Fax +45 4454 0111 Fax +33 1 56 05 23 23 Fax +49 64 41 29 41 55 Fax +39 02 574 03392 Fax +81 3 5421 2896 Fax +82 2 514 65 48 Fax +31 70 4132 109 Fax +852 2564 4163 Fax +351 21 385 4668 Fax +65 6773 0628 Fax +34 93 494 95 32 Fax +46 8 625 45 10 Fax +41 71 726 34 44 Fax +44 1908 609 992 Fax +1 847 405 0164 Order nos. [. . . ]