User manual LEICA MACROFLUO

[. . . ] Leica MacroFluoTM User manual Dear User, Thank you for your faith in our product. We hope your work with it is both enjoyable and successful. Leica MacroFluoTM with zoom 6. 3:1 or 16:1 are worldwide the only macro documentation systems for fluorescence. The MacroFluoTM concept combines the advantages of macroscopy ­ large fields of view, large working distances, parallax-free and precise imaging ­ with fluorescence technology (including multi color fluorescence) at a high resolution. [. . . ] 10), can also be used. Or: Fasten achromates to the fin focusing (4. 5) by means of the adapter. 2 6 4 3 3 1 7 5 Diaphragm ring At the bottom of the zoom system is a diaphragm ring (4. 4). The diaphragm ring must be removed if fine focusing is used. Fig. 4 Z6 APO on the left: Mount the objective on the diaphragm ring of the zoom system. Z16 APO on the right: Objective mounted on the fine focusing (diaphragm ring removed). 1 2 3 4 5 6 Z6 APO with zoom 6:1 Z16 APO with zoom 16:1 Objective Diaphragm ring Fine focusing 3 hexagon-head screws for fastening the diaphragm ring to the zoom system (the figure does not show all 3 hexagon-head screws) 7 3 hexagon-head screws for fastening the fine focusing to the zoom system (the figure does not show all 3 hexagon-head screws) Objective zoom system Fasten planapochromatic objective (4. 3) counterclockwise on the diaphragm ring of the zoom system (4. 4). Or: Fasten achromates to the diaphragm ring of the zoom system (4. 4) by means of the adapter. Objective fine focusing zoom system Loosen 3 hexagon-head screws (4. 6) at diaphragm ring (4. 4) and remove diaphragm ring. Tighten the fine focusing (4. 5) with 3 hexagonhead screws (4. 7) to the zoom system (4. 2). Fasten planapochromatic objective (4. 3) counterclockwise on the fine focusing (4. 5). 14 Leica MacroFluo ­ Assembly 2. 8 UV protection screen Fasten UV protection screen (5. 5) with clamp (5. 4) on the hinged arm (5. 1). Fasten hinged arm with UV protection screen to the carrier using the clamp (5. 2). 3 The hinged arm can be opened and closed via the central rotary knob (5. 3). In the opened state, all joints are freely movable and the hinged arm can be placed in any position. To block all hinged arms, turn the rotary knob clockwise. 5 4 2 1 5 Hinged arm with UV protection screen Always position the UV protection screen so that the operator can ever look directly onto the light spot (see p. 9). 1 Hinged arm 2 Clamp with clamping screw for fastening to carrier 3 Central rotary knob 4 Clamp with clamping screw for fastening to UV protection screen 5 UV protection screen Leica MacroFluo ­ Assembly 15 2. 9 Lamp housing The Leica lamp housing 106 z (11 504 069) is the light source for the incident illuminator LRF 4/22. It is used with 100-W mercury burners (11 500 138) or 103 W/2 (11 500 321) with extended life, the supply unit ebq 100 W and 103 W/2 (11 500 325) and straylight protection (10 446 271). 2. 10 Instrumentation of nosepiece disc The nosepiece disc can be fitted with a total of 4 filter blocks (filter system). 25) are available for various macro and multicolor applications (additional filter blocks upon request). The filter block offer from Leica Microsystems is continuously expanded with respect to the latest application requirements. The filter blocks (filter systems) consist of a combination of excitation filter, beam-splitting mirror and suppression filter. Please read ­ the separate operating instructions M2-216-1 and the safety instructions for the Leica lamp housing ­ the operating instructions and safety instructions for the supply unit ebq 100 ­ the operating instructions and safety instructions of the burner manufacturer and specifically its notes in reference to lamp breakage and the release of mercury. Fasten the stray-light protection to the lamp housing 106 z (1. 9) as described in the operating instructions M2-216-1. Fasten the lamp housing 106 z (1. 9) to the adapter component of the incident illuminator as described in the operating instructions M2-216-1. Connect the cable from the lamp housing to the supply unit ebq 100 W and 103 W/2. Power cable must meet country standards. [. . . ] ­ The excitation filter selects exactly those wavelengths from the beam selection of the light source that are used for exciting the fluorescence. This is made possible by highest transmissivity in the excitation range, while using the highest possible suppression effect in other wavelength ranges. ­ The dichromatic beam-splitting mirror reflects the short-wave excitation radiation to the specimen, while being transparent for the fluorescent light emitted from the specimen. ­ The suppression filter blocks excitation light dispersed by the specimen and entering in the objective. [. . . ]