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Manual abstract: user guide LEICA MM ANGIOGENESISBROCHURE
Detailed instructions for use are in the User's Guide.
[. . . ] Leica MM Angiogenesis
Analysis Software Drop-in for Leica MM AF
· Unique z-Series acquisition · Best focus algorithm for better quality result · Adaptive Background CorrectionTM for improved segmentation · Multi-Parameter analysis In many serious diseases, the human body loses control over angiogenesis. [. . . ] However, the surface is not flat and some tubules grow perpendicular to it. The Leica MM Angiogenesis Module for the Leica MM AF facilitates the acquisition and analysis of tube formation experiments.
powered by MetaMorph®
To capture the three dimensional behavior, the application module allows for a unique z-series acquisition. A best focus image is then obtained from the stack of images, and the analysis is run on that image. The module includes Adaptive Background Correction, adapting the vessel detection algorithm to the local intensity ranges outside the vessels to provide the most robust segmentation available. This technique enables vessel detection even with highly variable background fluorescence within a single image. The application module has a user-friendly interface to minimize set up efforts and provide the flexibility to customize the settings and measurements collected to better suit your needs.
Simple acquisition set up 1. Choose appropriate settings
Multi-parameter analysis Measurement options include: · Total tube length · Total tube area · Mean tube thickness · Number of segments · Number of branch points · Number of nodes · Total area of nodes · Mean node area
Easy configuration for analysis
Unique 3D acquisition, Angiogenesis Endothelial Cell Tube Formation Assay Courtesy of Min Wu, BD Biosciences. Select the contrast level
Customization through journaling Journals are sophisticated and powerful macros that record and perform a series of tasks without the need for a programming language. [. . . ] White: tubes, green: nodes.
Data export for further analysis
www. leica-microsystems. com [. . . ]
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