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Manual abstract: user guide LEICA MM CELL HEALTHDATASHEET
Detailed instructions for use are in the User's Guide.
[. . . ] Leica MM Cell Health
Analysis Software Drop-in for Leica MM AF
· Analysis of three fluorescent probes · Adaptive Background CorrectionTM for improved segmentation · Field and Cell-by-Cell data logging In some diseases, such as cancer, mutations may cause apoptotic pathways to malfunction, allowing uncontrolled proliferation of tumorigenic cells. [. . . ] Determine the intensity above local background 4. Specify the stained area (nucleus, cytoplasm or both) 6. Set the minimum and maximum cell width 7. Determine the intensity above local background 8. Optionally choose the reporting parameters
Multi-parameter analysis The Application Module can generate a broad range of parameters, including: · Counts of viable, early and late apoptotic, and necrotic cells · Percent of viable, early and late apoptotic and necrotic cells · Area, integrated and average intensity of nuclei
Powerful data export capabilities All measurements can be directly exported to a text file or Microsoft® Excel® for further analysis. "MetaMorph® is a Registered Trademark of MDS Analytical Technologies"
Interactive data display
Multiple probes acquisition Adherent Chinese Hamster Ovary (CHO-K1) cells were incubated with various concentrations of staurosporine. From top to bottom: control, 0. 1 M staurosporine, and 3 M staurosporine. BIOMOL International's Mit-E- Mitochondrial Permeability Detection (Hoechst 33342 + JC-1). [. . . ] Green: viable, blue: early apoptotic, purple: late apoptotic, red: necrotic.
Data export for further analysis
www. leica-microsystems. com [. . . ]
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