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Manual abstract: user guide LEICA MM COUNT NUCLEIBROCHURE
Detailed instructions for use are in the User's Guide.
[. . . ] Leica MM Count Nuclei and Cell Scoring
powered by MetaMorph® Analysis Software Drop-in for Leica MM AF
· Ideal for two-wavelength experiments · Count nuclei of most cell types · Adaptive background correctionTM for improved segmentation · Cell-by-Cell measurements Two popular and robust application modules are available together in Leica MM AF to facilitate easy and fast segmentation analysis. [. . . ] The second stain can label the nucleus, cytoplasm or both. The modules include Adaptive Background Correction, adapting the cell detection algorithm to the local intensity ranges outside the cells to provide the most robust segmentation available. This technique enables cell detection even with highly variable background fluorescence within a single image. A simple interface minimizes setup efforts and settings can be saved for future use or customized to fit your experiment.
Count nuclei analysis configuration Select a sample image to configure your settings
Easy configuration for analysis Count Nuclei setup is just two steps: 1. Select minimum and maximum nuclear size 2. Set the sample intensity level above the local background Cell Scoring setup involves a few easy steps:
Multi-parameter analysis Measurements for Count Nuclei include: · Count of nuclei · Integrated and average intensity · Total and mean area
Measurements for Cell Scoring include: 1. Choose the second image containing the positive marker stain 5. Select the stain area (nucleus, cytoplasm or both) 6. [. . . ] The modules can be incorporated into a Leica MM AF journal to increase the customization and automation of your analysis.
Cell scoring image analysis The module identifies the two different probes
www. leica-microsystems. com [. . . ]
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