User manual LEICA MZ10F

[. . . ] Leica StereoFluorescence Systems User Manual Contents Page Leica MZ10 F fluorescence stereomicroscope Description . Focusing drive, microscope carrier, MZ10 F optics carrier . 4 4 6 8 9 10 11 11 12 12 13 13 14 15 Operation FLUOIIITM filter system, light stop . 16 Adjusting the dioptric setting, focusing, observing . [. . . ] Connect the power cable to the supply unit and to the grid Make a note of the running-time meter reading . 1 4 25 3 Type Input voltage Inputfrequency Protection class Series no . Fuses ebq 100dc 90V to 250V AC, max 265VA 48Hzto63Hz IP20 G 34240090 The fuse holder is secured in the holder with spring clips on both sides . Insert a screwdriver behind the spring clips and extract the fuse holder . Insert the fuses (2×T3, 15AH250VF1/F2). 14 Refit the fuse holder Adjusting the mercury vapour lamp This adjustment is important for obtaining a uniform light spot and good-quality fluorescence . While adjusting the mirror image, do not project light on to the electrodes for long periods (risk of explosion through overheating) The two electrodes are imaged as an extension of the plane of symmetry of the discharge arc, but are difficult to see . Switch on the supply unit and wait for two or three minutes · The100Wsupplyunitemitsawhistle Swing out the UV-light excluder (page 21) · WorkwithouttheUV-lightexcluderisonlypermittedforadjustment, and then only for short periods Pull out the light stop (see page 16, MZ10 F and page 22, Fluorescence module) . MZ10 F: Turn the filter set in the beam path (page 16) . Stereo-fluorescence module: Insert the excitation filter (page 22) . Mark a cross on a piece of paper and place it in the middle of the illuminated spot Select the lowest magnification · Thedischargearcisvisibleintheilluminat dspotandlies e at about 45° to the lines of the cross (fig . a) Look into the eyepieces and focus on the cross Using your unaided eye, observe the discharge arc on the paper and bring it into focus with the focusing knob (3) (fig . a) Using the positioning knobs (1 and 2), displace the discharge arc (fig . b) Bring the mirror image of the discharge arc into focus with the knob (6) and use the knobs 4 and 5 to position it symmetrically relative to the original image (fig . c) · Forthe50Wburner, thedischarge-arcmageandits i mirror image should touch · Forthe100Wburner, thedischarge-arcmageanditsmirror i image should be superimposed . Using the focusing knob (3), readjust the illuminated field · Theilluminatedfieldshouldnowbelarge, circularand as uniform as possible Reposition the UV-light excluder correctly (page 21) . Hg50W Hg100W 3 Figs . a: Bringing the discharge arc into focus 1 Figs . b: Positioning the discharge arc relative to the cross 2 Figs . c: Focusing and positioning the mirror image of the discharge arc 4 5 6 15 Leica MZ10 F Dummy filter carrier In the dummy filter carrier there are two openings for the observation beam paths instead of the barrier filters . Use the dummy filter carrier: ­ if you are using less than four sets of filters ­ if you are working without the fluorescence illuminator for periods of up to 15 seconds . · Donotusethedummyfiltercarriertoblockthelightfromthe mercury-vapour burner for more than 15 seconds, or it will heat up . Light stop The FLUOIII filter system includes a slot for the light stop . In phases where the object is to be examined in e . g . [. . . ] Using the clamping screw, secure the clamp on the side-faced column, beneath the focusing drive . Filterholder fluorescence module The dichromatic beam splitter and the barrier filter are built into the fluorescence module in accordance with the configuration (see table, page 19) . The excitation filter is supplied in a separate filterholder . A filterholder with diaphragm is also supplied . This diaphragm can be slid into the illumination beam path if the object should not be illuminated . 3 12 Slacken the central fixing screw . [. . . ]