User manual LEICA TCS SP5 TANDEM SCANNER BROCHURE

[. . . ] Also the detection system must have a point-like sensitivity distribution. The detected signal is then again composed back into an image. For high speed recording the line-wise data acquisition is the limiting bottleneck caused by physical movement of the scanning mirror which is responsible for line-acquisition. [. . . ] Here, a new solution by Leica Microsystems solves the problem: Resonant Scanner The resonant scanning system of the Leica TCS SP5 works at 8000 Hz frequency and enables to record data in both directions of the scan motion. It thus acquires about 25 images per second at a frame resolution of 512 x 512 pixels. This is about 10 times faster compared to the recording speed of conventional scanners. For even faster events, one can reduce the number of lines per image managing up to 200 frames per second. For extremely fast changes, data can be recorded by images containing only one single line. These xt-scans allow up to 16. 000 Hz time resolution. Large field of view The application of resonant scanners has additional advances compared to other high-speed solutions. The field of view is very large (15 mm fov) compared to conventional scanners, where the field of view at 2000 Hz is restricted to some 3 mm fov. The field of view is tunable by resonant scanners in a large range. This does not work for systems that employ cameras (typically spinning-disk based systems or similar technologies with parallel illumination) since their field of view is fixed by the CCD chip size and the optical magnification. Ca2+ waves in muscle cells shown by a fluorescenct Calcium-indicator and recorded with a resonant scan system. Eisner University of Manchester, UK Zoom function by decreasing the scanning angle of galvanometer-mirror, pan function by adding a scanZoom 1 5 Pan offset in x and y to the (fov f iel f do With the computer controlled shifting of the y-position, the Leica TCS SP5 has a panning function also for the resonant scanner which allows for moving the position in the optical field. This is important for adjusting to a small section of the image field without cumbersome adjustment of the stage. This saves time and avoids the early damage of the sample. Drosophila melanogaster (eye section) vi e w ) zoom-image. Multicolor Imaging A further advantage of the resonant scanner is its integration into a confocal system with multiple channels and high efficient AOBS ® and SP ® detection for multi-parameter fluorescence experiments at high temporal resolution. Regarding this parallel beam systems (e. g. spinning-discs) have significant limitations, which can only be compensated by simultaneous recording with multiple cameras. A very expensive and complicated approach, where multiple in fact is restricted to two. The Leica TCS SP5 en passant offers effectively 5 channels simultaneously for high speed imaging. Beam Park But a resonant scanner cannot stand still at a given coordinate. [. . . ] research on structures of cytosceleton, organelles or tissues, where highest spatial resolution is required and the images should not show residual noise. On the other hand, physiology and biophysics, where naturally temporal resolution becomes very important and the images may, but must not necessarily look nice as long as data are recorded fast and safely. Of course, one can also create nice images with a resonant scanner by line- or frame averaging. [. . . ]