User manual LEICA TCS SP5 X BROCHURE

[. . . ] Leica TCS SP5 X X-citing Life ­ The Only Confocal That Tunes Into Any Excitation · · · · Ready for any dye the future brings The only system that fully adapts to the sample Full freedom and flexibility in emission AND excitation All you need for imaging ­ today and tomorrow! 22 Biological and medical research fluorescence imaging has evolved from studying one or two features in a specimen to examining even more features and their dynamic interactions in the cellular environment. This requires a variety of fluorescent dyes to study four, five, or more proteins simultaneously. As new dyes are developed and used, the ability to optimally excite and detect these fluorophores is necessary in both living and fixed specimen. [. . . ] Freedom to choose the specific excitation line in 1 nm increments allows optimal adjustment of the excitation to the special properties of the dye. This flexibility decreases damage to the cell and reduces unwanted cross-excitation. The Leica TCS SP5 X is the only confocal system that adapts to the research needs of today and tomorrow. "The White Light Laser offers a tremendous flexibility and will open our minds to new imaging applications. " Dr. Montoya Confocal Microscopy and Cytometry Unit Biotechnology Program Spanish National Cancer Center (CNIO) Madrid, Spain Leica Design by Christophe Apothéloz visible and ultraviolet radiation: 4 Intuitive Interface Allows Full Freedom and Flexibility for Scanning · Excitation lines can easily be selected to any wavelength with 1 nm accuracy · Tuning via the LCD control panel allows to select excitation light while scanning · Easy intuitive interface makes the Leica TCS SP5 X perfect for basic and advanced users · Interface allows easy access to all scanning parameters for both supercontinuum and fixed laser lines 5 · · · · AOBS® allows any combination of 8 laser lines ­ perfect for supercontinuum laser Greater sensitivity than any other confocal AOTF-controlled laser power Combination of fixed laser lines and supercontinuum lines together poses no problem Opening Doors and Opening Ready for any new dye of the future The number of available fluorescent dyes is growing tremendously. The ability to excite these dyes optimally with ideal spectral detection and minimal sample damage from high excitation sources is challenging. For most imaging applications the number of dye pairs available is endless, however, the ability to properly excite and control cross-excitation is a difficult procedure with the limited fixed laser lines available in today's laser scanning confocal systems. 120 ­ 100 ­ 80 ­ 60 ­ 40 ­ 20 ­ "The supercontinuum laser and AOBS are a perfect match. Full freedom of a white light laser could only be realized with the AOBS. The Leica TCS SP5 X saves experimental time to focus more on data analysis. " Prof. Alberto Diaspro Department of Physics University of Genoa Genoa, Italy 0 The TCS SP5 X perfectly adapts to any dye that will come today or tomorrow. With 200 nm of freely tunable excitation lines, the Leica TCS SP5 X can be tuned to provide optimal excitation of any available dye. This flexibility is critical for core facilities that service hundreds of users with various samples and various dyes. There will never be a need to turn away any sample or have difficult times exciting new dyes. 6 Minds A new dimension to spectral separation New GFP mutants are all perfectly separated by excitation mapping with the Leica TCS SP5 X · · · · · Cy2, exc 491 nm, emis 499 ­ 505 nm, NMDA receptors Lp, exc 474 nm, 509 ­ 514 nm, beads in vacuols Acridine Orange, exc 484 nm, emis 520 ­ 532 nm, nucleus Cy3, exc 551 nm, emis 555 ­ 575 nm, Gaba-B-R1 receptors TexasRed, exc 562 nm, emis 605 ­ 612 nm, vacuoles and endosomes Alexa 594, exc 594, emis 615 ­ 625 nm, nuclear and cellular membrane Alexa 633, exc 633 nm, emis 655-665 nm, clathrine vesicles Cy5, exc 640 nm, emis 661-675 nm, cilia Up to 5 spectral detection channels Tune to exact spectral range while scanning Almost no light loss from first to last channel Cooled PMTs allow for greater sensitivity and less noise Never worry about barrier filters again Best red emission detection available Emission and excitation scans allow for new dimensions in spectral imaging · · · 2 years of scanning light guaranteed Paramecium stained with 8 fluorescent dyes. Excitation and emission lambda scans were obtained to determine optimal imaging conditions for each dye. of Physics, University of Genoa, Italy Most confocal systems on the market claim to have some capability to "spectrally" separate dyes. The Leica TCS SP5 X allows the use of emission scan dye separation and excitation scan dye separation for a new dimension in spectral imaging. This technology opens new doors to confocal microscopy applications including excitation AND emission mapping that can be used for new dye development, or existing applications such as FRET. "With the TCS SP5 X we are solving problems we did not know existed. " Dr. [. . . ] From patient to pathologist, the range includes the ideal product for each histology step and high-productivity workflow solutions for the entire lab. With complete histology systems featuring innovative automation and NovocastraTM reagents, the Biosystems Division creates better patient care through rapid turnaround, diagnostic confidence and close customer collaboration. People's Rep. of China: Hong Kong · Surgical Division The Leica Microsystems Surgical Division's focus is to partner with and support micro-surgeons and their care of patients with the highest-quality, most innovative surgical microscope technology today and into the future. and representatives of Leica Microsystems in more than 100 countries. www. leica-microsystems. com [. . . ]