User manual LEICA TCS SPE BROCHURE

[. . . ] Technical Documentation Leica TCS SPE · Spectacular Imaging · Easy to Achieve · Built-in Reliability · Affordable Excellence The new high resolution spectral confocal Leica TCS SPE is an integrated system for life science research. A reliable partner, providing spectacular results easily. A 2 7 6 15 3 14 1 4 13 12 2 1 B 2 14 4 6 7 8 16 13 12 11 15 5 3 10 1 C 2 1 14 7 6 2 3 8 9 16 12 13 10 4 11 3 5 15 4 5 6 7 8 9 10 11 12 13 14 15 16 Research microscope A DM2500 CSQ B DMI4000 CSQ C DM5500 CSQ Confocal scan head Supply unit, including 3 or 4 lasers and power supply SuperZ Galvo stage Microscope control unit Camera port Digital camera EL6000 fluorescence illumination control Smart move microscope control Computer table Anti-vibration microscope table Computer mouse Keyboard Monitor Workstation Control panel 3 Specifications Leica TCS SPE-II Scan Head Scanner Method Confocal channels Scanner Sequential scan Channels Range (min - max) Scan formats Image depth Spectral detection Type Spectral resolution Bandwidth [pixel] [pixel] [bit] True Confocal Point Scanner 1 Galvo, [x, y] yes 1 ­ 8, sequential multiplexing 1282 ­ 20482 128, 256, 512, 1024, 2048 8 or 12, switchable yes continuously variable 5 nm 430 - 750 Resolution Spectral Detection [nm] [nm] Detector Detector Detector type Detector connection Illumination 1 ultra high dynamic PMT direct laser Pinhole Pinhole type Range (min - max) Pinhole adjustment Control [µm] [%] motorized, variable 35 ­ 600 0 ­ 100 automated via GUI Beam Splitter Type Beam splitter wavelength (532nm laser systems) Beam splitter wavelength (561nm laser systems) ND-splitter for TLD/ Beam splitter exchange [nm] [nm] [%] high performance dichroics 405/488/633 405/532 405/488/561/635 488/561 30/70 automated continuously variable 1x ­ 58x 0. 1 xt xyz; xyz xyt; xzt xy; xz xyzt; xzyt xyt; xzt xyz xyzt 15. 5 Zoom Zoom type Zoom range Zoom increment 2D 3D Time Lambda Time Time & lambda Lambda Time & lambda [mm] Scan Modes FOV Field of view (diagonal) Speed Speed mode Line speed range max @ 1282 bi-directional standard @ 5122 min @ 20482 [Hz] [f/s] [f/s] [f/s] uni-, bi-directional 400, 600, 800, bi: 1200, 1600 6. 1 2. 2 0. 8 4 Supply Unit Laser Laser type Laser Laser excitation wavelength Excitation attenuation Excitation attenuation control Range [nm] [%] solid state max 4 405, 488, 532, 561, 635 AOTF, direct modulation automated 0 ­ 100 Computer External PC Processor Memory HD-Size Operating system USB FireWire Ethernet Serial Mouse Keyboard [GB] [GB] yes Intel Core i7 4 500 Windows 7 8 4(2x 1394a, 3x 1394b) 1 0 optical yes Interfaces Monitor Graphics resolution Monitor [Pixel] 1280 x 1024, 2560 x 1600 (optional) 1x 19'', 2x 19'' (optional), 30'' (optional) Power Supply Power supply integration Type Voltage range Z-focus Z-resolution Z-focus device, other Leica DFC360 FX Digital Camera Leica DFC310 FX Digital Camera Leica Roper CoolSNAP HQ2 Camera Leica Hamamatsu 9100-02 Camera upright inverted [V] yes autoselect 100 - 240 galvanometer stage 10 [nm] microscope drive DM5500 yes yes yes yes DM2500 CSQ DM5500 CSQ DMI4000 CSQ LIF, TIFF, AVI, JPEG, MOV yes yes yes yes yes yes yes yes yes 10 ­ 80 18 ­ 30 23°C +/- 2°C Z-Drive Digital Cameras Support Microscope Types Software Export Modules Formats LAS AF SPE Live Data Mode LAS AF SPE Colocalization LAS AF SPE Deconvolution LAS AF SPE 3D Visualization LAS AF SPE Dye Finder LAS AF SPE Core Review Software LAS AF SPE Camera Advanced LAS AF SPE FRAP LAS AF High Cont. [. . . ] 9b 9a 9b 15 6604 410 Stage Insert for SuperZ, rotatable 15 5935 411 Stage Insert for SuperZ, universal 15 6604 412 Stage Insert for SuperZ, basic (system included) Motorized xy-Stage Kits SuperZ Galvo Stage Inserts 2 9b Optical Outfit DIC for Microscopes 3 4 5 15 5931 251 Optical outfit DIC for DM 2500 CSQ 15 5931 551 Optical outfit DIC for DM 5500 CSQ 15 5933 451 Optical outfit DIC for DMI 4000 CSQ Digital Camera Kit 8 9b 156605303 Leica DFC360 FX Digital Camera Kit SPE 15 6605 304 Leica DFC310 FX Digital Camera Kit SPE 15 6605 305 Roper CoolSNAP HQ2 Camera Kit SPE 15 6605 306 Hamamatsu 9100-02 Camera Kit SPE DIC Prism Sets for Basic Objective Sets 18 15 5934 152 15 5932 150 15 5934 150 19 15 5932 154 15 5932 152 15 5934 155 DM2500 Prism Set Basic DM Prism Set Basic DMI Prism Set Basic Plate Inserts 15 6604 420 Plate Insert -Microtiter Plate 15 6604 421 Plate Insert -Willco Dish 35 mm 15 6604 422 Plate Insert -Willco Dish 50 mm 15 6604 423 Plate Insert -MatTek Dish 35 mm 15 6604 424 Plate Insert -Petri dishes 36 mm 15 6604 425 Plate Insert -Petri dishes 39 mm DM2500 Prism Set Basic V-VIS DM Prism Set Basic V-VIS DMI Prism Set Basic V-VIS 6 Confocal Systems RGB/RYB ccessories: ent ternal light source HXP120 burner 16 15 66 01 202 Leica TCS SPE w. DM2500 RGB 15 66 01 502 Leica TCS SPE w. DM5500 RGB 15 66 01 402 Leica TCS SPE w. DMI4000 RGB 15 66 01 203 Leica TCS SPE w. DM2500 RYB 15 66 01 503 Leica TCS SPE w. DM5500 RYB 15 66 01 403 Leica TCS SPE w. DMI4000 RYB HC-Objectives for RGB/RGBV Systems 16 17 18 155935200 Basic Objective Set HC 10x, 40x, 63 15506285 HC PL APO 10x/0. 40 CS 15506326 HC PL APO 20x/0. 70 Imm/Corr CS 15506251 HCX PL APO 40x/1. 25 Oil CS 15506188 HCX PL APO 63x/1. 40 Oil CS 15506279 HCX PL APO 63x/1. 20 W Corr CS 15506194 HCX PL APO 63x/1. 30 Glyc Corr CS 21 Confocal Systems RGBV/RYBV Liquid light guide Adapter, Filter, Oculars 15 66 01 220 Leica TCS SPE w. DM2500 RGBV 15 66 01 520 Leica TCS SPE w. DM5500 RGBV 15 66 01 420 Leica TCS SPE w. DMI4000 RGBV 15 66 01 221 Leica TCS SPE w. [. . . ] 15 66 01 430 Leica TCS SPE DMI4000 Confocal Prep. 10 15 Confocal Scanning Units 15 66 01 702 15 66 01 703 15 66 01 720 15 66 01 721 Leica TCS SPE Scanning Unit RGB Leica TCS SPE Scanning Unit RYB Leica TCS SPE Scanning Unit RGBV Leica TCS SPE Scanning Unit RYBV ACS Objectives for RGBV/RYBV Systems 16 17 17 19 155935210 Basic ACS Objective Set 10x, 40x, 63x 15507902 ACS APO 10x/0. 30 Dry CS 15507904 ACS APO 20x/0, 60 Imm CS 5 BF 11 15507901 ACS APO 40x/1. 15 Oil CS 15507900 ACS APO 63x/1. 30 Oil CS Climate Control 15 6605 602 Universal Gas Cover for SuperZ w. In/Outlet 9b 9c Hardware-Options 13 15 6605 711 Control Panel for TCS SPE 15 6605 702 Second 19"-TFT Monitor for TCS SPE 15 6605 703 Eizo 30" high brilliance monitor TCS SPE Software LAS AF - Screening TCS SPE HCS A High Content Screening Automation 14 LEICA HCS A for TCS SPE-II see LAS AF Matrix Products Software LAS AF - Imaging TCS SPE 14 15 6602 202 LAS AF SPE Live Data Mode 16 6022 203 LAS AF SPE Co-Localisation 15 6605 600 TCS SPE CO2 Control Set 110V/230V 9c Including: SuperZ CO2 Cover CO2 Controller, Humidifier Tables 11 15 5934 190 Microscope Table for Upright and Inverted Systems, passive 15 6606 190 Computer Table 15 6602 204 LAS AF SPE Deconvolution 15 6602 205 LAS AF SPE 3D Visualisation 15 6602 208 LAS AF SPE Dye Finder 15 6602 211 LAS AF SPE Camera Advanced 15 6602 215 LAS AF SPE FRAP 15 6605 400 Climate Chamber for DMI 4000 CSQ Including Heating & Temp Control Unit 10 12 7 Room Requirements Power Supply Power supply integration Type Voltage range Power consumption Independent circuits Frequency Fuse: standard Humidity (noncondensing) Operating temperature Guaranteed stability Confocal unit, max Microscope, max System Static floor load [V] [VA] [no. ] [Hz] [A] [%] [°C] yes autoselect 100 - 240 800 1 50/60 10 10 - 80 18 - 30 23°C +/- 2°C 75 45 120 150 Environment visible radiation: Load Capacity and Weight [kg] [kg] [kg] [kg/m2] 1450 mm 900 mm 100 mm 740mm 1300 mm 720 mm 700 mm 2200 mm 1400 mm 900 mm 100 mm 740mm 1300 mm 720 mm 900 mm 2400 mm www. leica-microsystems. com LEICA and the Leica Logo are registered trademarks of Leica Microsystems IR GmbH. 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