User manual LEICA Z16 APO A

[. . . ] Leica Z6 APO A Leica Z16 APO A User Manual General Instructions Safety Concept Before using your macroscope for the first time, please read the "Safety Concept" brochure included with your instrument. It contains additional information about handling and care. Use in clean rooms The Leica Z series can be used in clean rooms without any problems. Cleaning Do not use any unsuitable cleaning agents, chemicals or techniques for cleaning. [. . . ] Tighten the screw of the limit stop. Injuries when manipulating the specimen because the fingers or hand get pinched. Accidental contact between the objective and the specimen, and potential resulting damage. Readjusting the motorized focus The motorized focus is factory-adjusted and normally does not need to be readjusted-- even if the maximum travel path is changed. Exception: If the power fails while the motorized focus is moving, the position data are lost. In this case, the calibration must be repeated using the Leica LAS software or the Leica SmartTouchTM. To do so, please consult the respective manual. Leica Z6 APO A / Z16 APO A User Manual 42 Cables: Diagram 1/3 Z6 / Z16, APO & APO A with Z-Series DCI-Box PC CTL2 Power plug CTL2 CTL1 Z-Series DCI -Box CTL2-Ports Z6 / Z16 APO A Connection between serial- and CTL1-Port ** Powersupply 10 450 266 * ** *** SmartTouchTM * Footswitch*** UMC Serial connection loop possible Adapter-cable 10 447 401 needed Serial connection of up to 5 footswitches possible Leica Z6 APO A / Z16 APO A User Manual 43 Cables: Diagram 2/3 Z6 / Z16, APO & APO A with interface on HighPerformance-M-Series-Column Z6 / Z16 APO A PC Z-Series DCI-Box CTL2 CTL2 CTL1 Interface on Focus Column TL RCITM USB CTL2-Ports Do not use CTL1-Port and Power Plug when DCI-Box is connected to the motor focus column! Power Supply * ** *** **** Serial connection loop possible Serial connection of up to 5 footswitches possible For motorfocus only Not with new motorfocus SmartTouchTM * Footswitch** Handwheel*** 10 450 298 UMC**** Leica Z6 APO A / Z16 APO A User Manual 44 Cables: Diagram 3/3 Z6 / Z16, APO & APO A with TL RCITM Base and IsoProTM Stage Do not use CTL1-Port and Power Plug when DCI-Box is connected to the motor focus column! PC Z-Series DCI-Box CTL2 CTL2 CTL1 Interface on Focus Column CTL2 DCI Controlbox Power CTL2 CTL2 CTL2 USB USB CTL2 Power Supply TL RCITM Z6 / Z16 APO A Alternative installation Please use the Leica Application Suite to configure the functions of the system. CTL2 CTL2 LED3000 NVI Leica Z6 APO A / Z16 APO A SmartTouchTM User Manual Smart Move PSC Connector from IsoProTM Stage 45 Leica LED3000 NVITM (Near Vertical Illumination) Required tools None Installing the Leica LED3000 NVITM 1. Connect the Leica LED3000 NVITM to the focusing column via the CTL2 connection if the focusing column is equipped with integrated electronics. Alternative installation for manual columns 1. Connect the external power supply unit (10 450 266) to the Leica LED3000 NVITM. Place the Leica LED3000 NVITM on the objective from below and tighten the locking screw. diameter of 58 mm. with any objectives that have an outer The Leica LED 3000 NVITM can be used between 50 mm and 150 mm. The supported working distance is Leica Z6 APO A / Z16 APO A User Manual 46 Quick Start Guide Leica Z6 APO A / Z16 APO A User Manual 47 Overview of an automated Leica Z Series Macroscope (Leica Z16 APO A) 1 2 3 4 5 6 7 8 9 10 Digital camera Photo/videotube Eyepieces Video/Phototube Y Microscope carrier Connection for control cable Optics carrier (zoom body) Objective LED ring illuminator IsoProTM motorized mechanical stage 11 SmartTouch control unit 12 Motorized microscope column 1 2 3 4 5 6 7 8 10 12 9 11 Leica Z6 APO A / Z16 APO A User Manual 48 Eyepieces Leica Z6 APO A / Z16 APO A User Manual 49 Magnification Factors of the Eyepieces An eyepiece not only makes it possible to look passively into the macroscope, but also has a critical effect on the maximum magnification. The magnification factor is between 10× and 40×. The following eyepieces are available for the Z series: Magnification 10× 16× 25× 40× Dioptric Correction ± 5 diopter settings ± 5 diopter settings ± 5 diopter settings ± 5 diopter settings Order number 10 450 023 10 450 024 10 450 025 10 450 026 Leica Z6 APO A / Z16 APO A User Manual 50 Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer. Simply push them into the tube and they are ready to use. Each eyepiece offers a certain magnification factor that has a determinative effect on the total magnification. Furthermore, all Leica eyepieces can be equipped with practical graticules that enable measuring and quantifying of specimens. Dioptric Correction A built-in dioptric correction is available for eyeglass wearers. If an eyepiece is equipped with the integrated dioptric correction, turn the value to the "0" mark. If an eyepiece is equipped with the integrated dioptric correction, turn the value to the "0" mark. By the way, one benefit of viewing with eyeglasses is a drastically lower risk of bacterial transmission (see page 53). The soft material of the eyecup also ensures that your glasses will not be scratched, even if they contact the eyepiece. Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise. Hold the eyepiece firmly and rotate the eyecups clockwise towards the rear, as otherwise the viewing distance is too great. Leica Z6 APO A / Z16 APO A User Manual 51 The Correct Interpupillary Distance The interpupillary distance is correctly set if you see a single circular image field when looking at a specimen. If you are still a novice macroscope user, you may need a short time to become accustomed to this. [. . . ] aperture Yes*** Yes*** Remote control options Yes**** Yes AntiShockTM Pads Yes Yes Dimensions of base (W×H×D) 340×390×95 mm 340×440×95 mm *Single-sided **With cold light source Leica CLS150 LS ***Concave mirror ****With external light source Leica Z6 APO A / Z16 APO A User Manual 107 Leica IsoProTM Motorized XY Stage Compatibility Stage size (L × W × H) Travel Speed X, Y Motor resolution X, Y Repeatability X, Y Positioning accuracy X, Y Hysteresis X, Y Motor Backlash Adapters Acceleration Computer connection Control options Power supply Leica incident-light and TL bases (TL BFDF, TL RCTM, TL RCITM) 335. 5 mm × 370 mm × 41. 5 mm 152 mm × 102 mm (6" × 4") 30 mm/s 0. 25 µm ± 2 µm ±20 µm over the entire positioning range 30 µm Direct current (DC) motor with encoder Slack-free All adapters for TL bases S-shaped acceleration USB Leica PSCTM, Leica Smart Move, Leica LAS via PC Input: 100­240 VAC 50 / 60 Hz 1. 0 A Output: 15 VDC 2. 66 A 40W max. Leica Z6 APO A / Z16 APO A User Manual 108 Appendix Leica Z6 APO A / Z16 APO A User Manual 109 Calculating the Total Magnification and Field of View Diameter Parameter MO ME z q r NFOV Magnification of the objective Magnification of the eyepiece Magnification of the changer position Tube factor, e. g. 1. 5× for coaxial incident light, 1. 6× for 45° ErgoTubeTM Factor of 1. 25× when using video/phototube Y Field number of the eyepiece. Field numbers are printed on the eyepieces: 10× = 21, 16× = 14, 25× = 9. 5, 40× = 6. Example MO ME z q r 1× objective 25×/9. 5 eyepiece Zoom position 4 Coaxial incident light 1. 5×, tube factor Factor 1. 25× Calculation example: Magnification in the binocular tube: MTOT VIS = MO × ME × z × q × r or 1 × 25 × 4 × 1. 5 × 1. 25 = 187. 5× Calculation example: Field of view diameter in the specimen OF: NFOV MO × z ×q ×r Leica Z6 APO A / Z16 APO A User Manual 110 Care, Maintenance, Contact Persons We hope you enjoy using your high-performance macroscope. [. . . ]