User manual MICROCAL VP-ITC

[. . . ] Binding Stability Kinetics MicroCal, LLC 22 Industrial Drive East Northampton, MA 01060 USA Phone: +413 586 7720 Fax: +413 586 0149 support@microcalorimetry. com VP-ITC MicroCalorimeter User's Manual MAU130030 Rev. E MicroCal, LLC VP-ITC MicroCalorimeter User's Manual Table Of Contents General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i How To Use This Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . i How to Get Help from MicroCal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . ii Symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iii Operators Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . iv Product Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Instrument Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . v Safety Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . vi European Users: CE Mark . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] The auto-pipette will move the plunger of the injection syringe till the Teflon tip is just above the filling port of the syringe. You should hear a beep when the movement is complete. Attach the tube of the plastic filling syringe to the filling port of the injection syringe. · · MAU130030 Rev. E 44 MicroCal, LLC VP-ITC MicroCalorimeter User's Manual · · Slowly withdraw the plunger of the plastic filling syringe to draw up the titrant solution until you see the solution exit through the top filling port. Click on the Close Fill Port button as soon as you see the liquid exit the top filling port. The pipette will lower the plunger of the injection syringe till the white Teflon tip is completely below the filling port (ca. You should here a beep when the movement is complete. Remove the hose of the plastic filling syringe from the filling port of the injection syringe. The auto-pipette will depress the plunger of the filling syringe to inject the sample back into the filling test tube, then raise the plunger to refill the injection syringe. When the movement is complete the tip of the injection syringe will be positioned to its original position, just below the filling port. A beep will indicate the completion of the movement. Click on the Purge->ReFill button again to repeat the purge/refill action. The purpose of both purge/refill procedures is to dislodge any air bubbles from inside walls of the injection syringe (which may have occurred during the first filling) and expel them back into the titrant solution. Carefully remove the pipette from its stand by picking it straight up until the glass barrel of the injection syringe is above the top part of the pipette stand. Carefully move the pipette so that it is directly above the center positioned sample cell access tube (this is the hole on the right). Carefully insert the pipette into the sample cell access tube. Watch the end paddle of the long needle to insure it is inserted directly into the access hole, hold the pipette vertical and slowly lower the pipette. When the pipette is almost completely inserted you may have to push down slightly to compensate for the resistance of the rubber o-ring to seat the pipette. · · · · · · Precautions with Pipettes Never assemble or disassemble the pipette/syringe assembly when the pipette screw is not in its home (open port) position. Failure to raise the pipette screw prior to assembling/disassembling the assembly will likely bend the pipette screw beyond repair. The long needle and stir paddle on the pipette injection syringe must not be bent even slightly or baseline stability will be compromised. Exercise care at all times in the handling of the pipette syringes. When removing the pipette from the instrument after an experiment ends ensure that the needle is concentric with the access tube until the end of the stir paddle is clear of the hole. [. . . ] If you double click on this numeric value the display will change to a time function. 93 MAU130030 Rev. E MicroCal, LLC VP-ITC MicroCalorimeter User's Manual 7. 2 Customizing VPViewer In instances of multiple users operating the same VP-ITC system, it may be advantageous for those users to be able customize some aspects of the VPViewer application and recall those custom settings. Specifying the desired VPViewer configuration file at start up can do this. If no configuration file is specified then the default configuration file will be used. [. . . ]