User manual SANYO MCO-19AIC BROCHURE

[. . . ] SterisonicTM GxP Series Cell Culture CO2 Incubators MCO-19AIC(UVH) MCO-19AIC(UV) MCO-19AIC The industry's most complete cell culture solution for highly regulated applications or conventional incubation. Now with safe, effective and documented two-hour in situ H2O2 sterilization for fastest decontamination turn-around and maximum availability. SANYO SterisonicTM GxP Model MCO-19AIC(UVH) with integrated H2O2 sterilization system. General Features & Benefits The SANYO SterisonicTM GxP incubator is designed for a wide array of demanding and highly regulated applications in the biomedical, pharmaceutical, medical research and clinical laboratory. Representing years of research, development and component testing, the SterisonicTM GxP incorporates a collective of mutually functional systems and design attributes to offer a holistic solution to cell culture protocols, from the most sophisticated to more familiar and conventional processes. SterisonicTM GxP Selection H2O2 Vapor Sterilization SafeCellTM UV Decontamination Single Beam, Dual Detector IR CO2 Sensor MCO-19AIC(UVH) Standard Standard Standard MCO-19AIC(UV) Optional Standard Standard MCO-19AIC Optional Optional Standard Sterilization and Decontamination · The unique SterisonicTM GxP H2O2 sterilization system limits downtime to less than three hours when total chamber sterilization with verification is desired. · All interior components and CO2 sampling loop are sterilized in situ; no need for removal and autoclaving. · Active Background Contamination ControlTM fights contamination while cell culture protocols are in process. [. . . ] The H2O2 atomizer and cable can be disconnected and removed and all interior components restored to their normal position. 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Finish. Incubator must cool from high heat temperatures to near ambient. 5 Design & Technology Dual Wavelength Infrared CO2 Control System The SANYO single beam, dual detector infrared CO2 system offers unprecedented control accuracy and stability by simultaneously measuring two wavelengths. The chamber sample and a reference is configured around a ceramic-based sensor linked to the microprocessor controller with a sophisticated P . I. D. /RTM (proportional, integral and derivative) algorithm. Benefits include ultra-fast recovery without overshoot and accurate CO2 averages during periods of frequent incubator access with multiple door openings. · The ceramic-based technique is impervious to changes in temperature and relative humidity, highly stable during door openings, and sensitive to restoration of setpoint equilibrium when the door is closed. · The sensor maintains a continuous auto-zero function for accuracy. · Actual CO2 is displayed on the main control panel. · A CO2 sample port mounted on the incubator front permits convenient confirmation of chamber CO2 density. · An optional automatic CO2 switchover system is available; see Accessories. A two-stage regulator from the supply cylinder to the incubator is required; see Accessories. SafeCellTM UV System Sterisonic GxP Models ­UVH and ­UV include SafeCellTM UV contamination control technology based on an integrated combination of narrow bandwidth, ozone-free ultraviolet light, ceramic infrared CO2 control, InCu SaFeTM copper-enriched stainless steel alloy interiors and Direct Heat and Air JacketTM heating managed by a microprocessor controller. · Trace contaminants that attach to interior surfaces are destroyed by the passive germicidal properties of the InCu SaFeTM surfaces. H2O2 Sterilization The unique SterisonicTM GxP H2O2 sterilization system limits downtime to less than three hours when total chamber sterilization is desired. · All interior components and CO2 sampling loop are sterilized in situ; no need for removal and autoclaving. · Available as standard on the MCO-19AIC(UVH); optional on the MCO-19AIC(UV). · Following sterilization, SANYO Active Background Contamination ControlTM fights contamination while cell culture protocols are in process. · When components are removed, all interior surfaces are exposed for conventional wipe down. · Large curve corners and electropolished surfaces are easy to clean. · Pass thru ports accommodate probes or instrumentation leads as required for specialized cell culture protocols. Each chamber includes a port positioned in the interior, rear wall, upper left, with dual, non-VOC silicone stoppers inside and outside the cabinet for added protection. Mycoplasma Survival Results Chart summarizes test results with four strains of mycoplasma. Results demonstrate how SANYO InCu SaFeTM copper enriched stainless steel alloy offers germicidal properties of conventional C1100 copper while maintaining both corrosion-proof and discoloration-resistant properties of conventional Type 304 stainless steel. Detailed test results are available from SANYO. InCu SaFeTM Construction for Germicidal Protection SANYO offers exclusive use of InCu SaFeTM copper-enriched stainless steel alloy interior surfaces within a technical design created to eliminate contamination sources and to mitigate the effect of airborne contaminates introduced through normal use. · Selected to provide natural germicidal protection without rust or corrosion, InCu SaFeTM expresses a natural germicidal attribute to inhibit the growth of molds, fungi, mycoplasma and bacteria. · All interior components, including the air management plenum, shelf supports, humidity pan and blower wheel assembly are easily removable without tools if required. · During the H2O2 sterilization cycle interior components can be repositioned within the chamber for in situ sterilization. SANYO InCu SaFeTM Conventional Type 304 Stainless Steel Positive Control Mycoplasma Strain Mycoplasma fermentans PG18 Mycoplasma orale CH19299 YES Mycoplasma arginini G230 Mycoplasma hominis PG21 NO YES SafeCellTM UV Mode After H2O2 Vaporization Function The UV lamp automatically cycles ON for up to 90 minutes following the seven-minute H2O2 vapor cycle, reducing the H2O2 to water droplets. These droplets automatically condense onto a naturally cooler section of the interior floor for easy wipe-up. [. . . ] No moving parts; airflow passes over in/out ports to sustain Venturi effect, continuous Single point zero automatic; semi-automatic one point span @ reference optional. Gentle vertical airflow, continuous with inner door closed. 95%RH @ 37°C through evaporation via DHATM heating system; reflective optical low water sensor. Control, Monitoring, Alarm Temperature and CO2 Control Display Data Acquisition Communications P . I. D, setpoint resolution 0. 1°, 0. 1% Alphanumeric LCD digital display messaging. Automatic log function of temperature and CO2. [. . . ]