User manual ZEISS 16

[. . . ] . . . . 11 5. 1 Universal rotary stage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5. 1. 1 Sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5. 1. 2 Schmidt ruler. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5. 1. 3 Segments. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5. 2 Objectives. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11 5. 3 Condensers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 5. 4 Wulff network . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12 6 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13 7 Literature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 8 Wulff network for stereographic projections . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15 B 40-017 e / 12. 96 1 1 Notes Operation!The instrument may be operated by trained personnel only who must be familiar with the possible dangers involved in microscopy and the relevant field of application. Dust!Dust and dirt can impair the performance of the instrument. Therefore, protect the instrument against these interferences as far as possible. [. . . ] Use dust caps to close the vacant nosepiece eyes. Check Table 2 B 40 - 017 e / 12. 96 5 4 Instructions for use 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 Vernier A2 Schmidt ruler Vernier A1 Clamping screw A5 Click stop mechanism A5 Pins for vertical adjustment of lower segment (4x) Arresting pin universal rotary stage Control for condenser height adjustment Vertical condenser stop Centering screw for condenser Dovetail for condenser Aperture diaphragm Condenser Click stop mechanism A2 Centering screw for condenser Clamping screw for condenser Clamping screw for univ. rotary stage Click stop mechanism A4 Vernier A4 Threaded bore for stage clamp Clamping mechanism A2 Screw for upper segment Lower segment Upper segment Annular bearing Clamping device A4 Fig. 3 Universal rotary stage for Axiolab Pol 6 B 40 - 017 e / 12. 96 4 Instructions for use 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 Vernier A2 Schmidt ruler Vernier A1 Screw for mounting univ. rotary stage Click stop mechanism A5 Clamping screw for stage centering Rotary stage of microscope Clamping screw A5 Adjusting screw for stage centration Mounting screw for univ. rotary stage Adjusting screw for stage centration Clamping screw for stage centration Click stop mechanism A2 Click stop mechanism A4 Vernier A4 Threaded bore for stage clamp Clamping mechanism A2 Screw for upper segment Lower segment Upper segment Annular bearing Clamping mechanism A4 Fig. 4 Universal rotary stage for Axioplan Pol B 40 - 017 e / 12. 96 7 4 Instructions for use 4. 2 Preparation of universal rotary stage 4. 2. 1 Positioning the sample Select the suitable segment pair (Fig. 1/6 and 7), The refractive indices of the sample and the segment pair should be almost identical. For quartz or feldspa crystals, for example, the segment pair featuring the refractive index nD = 1, 556 should be selected, for pyroxen or amphibol crystals the one with the refractive index nD = 1, 648. Position the sample and put one drop of immersion fluid on it. 1/6) parallel to the sample so that the screws (Fig. 3/22) touch the threaded bores in question. to measure the optical axes (dark position when tilted about the A4 axis). If only one of the axes of a biaxial crystal lies between the tilting range A4, the angle of the optical axes must be determined using the Wulff network (Fig. 5). B 40 - 017 e / 12. 96 9 4 Instructions for use 4. 3. 3 Working in circularly polarized light. The Axiolab Pol, Axioplan 2 Pol and Axiophot 2 Pol microscopes permit the universal rotary stage to be used also for work in circularly polarized light. For this, the circular polarizer D consisting of a polarizer and two /4 plates must be used. The polarizer and one of the /4 plates is used in the place of the standard polarizer. The second /4 plate is inserted in the compensator mount. Before doing so, adjust the polarizer and the analyzer so that they are at a 90° angle to each other. Align the upper /4 plate to the lower one until dark position is reached without adjusting the objective. Circularly polarized light can be used in conjunction with the universal rotary stage to precisely measure the angle of the optical axes 2 V in birefringent, optically biaxial crystals, for example. If you wish to work in linearly polarized light, remove the two /4 plates from the beam path. For details on the use of circularly polarized light, please refer to Zschach [15]. Note: Example 3: Measuring the angle 2 V of the optical axis of a biaxial crystal in circularly polarized light Proceed as described in example 2 (steps 1 to 10). Switch in the Bertrand lens (focus Axioplan microscopes). [. . . ] It consists of the stereographic projection of meridians and latitudes onto a horizontally oriented plane. If the measurement of the axis angle of optically biaxial crystals, for example, reveals one of the axial directions to lie outside of the tilting range of the A4 axis, the stereographic projection can be used to determine the second axial direction and thus the axis angle 2 V of the crystal under examination. 12 B 40 - 017 e / 12. 96 6 Maintenance For general information on care and maintenance, please refer to the operating instructions G 42-311, B 40-016 and B 40-042. Kindly also observe the following:: The universal rotary stage is a precision instruTreat the segments with care (risk of scratchGlycerin ing). ment and must therefore be protected against mechanical damage to ensure that settings and adjustments are not lost. Always clean the universal rotary stage imme- is recommended as an immersion agent due to it being water soluble. For this, loosen the knurled screws of the upper segment and lift it out of the center part of the universal rotary stage together with the sample and the lower segment. [. . . ]