User manual ZEISS AXIOLAB A

[. . . ] Axiolab A Reflected-Light Microscope Operating Manual Carl Zeiss Axiolab A Knowledge of this instruction manual is necessary for device operation. Please get familiar with its contents and especially the precautions for safe device operation. Changes due to further technical development are reserved; this manual is not covered by an update service. © Unless expressly authorized, forwarding and duplication of this document, utilization and communication of its contents are not permitted. [. . . ] · Insert the fixed eyepiece, e. g. E-PL 10×/20 Br. , in one tube and the focusing eyepiece E-PL 10×/20 Br. The focusing eyepiece is used to compensate for ametropia of the user's eyes. 2. 3. 1 Insertion of Eyepiece Reticle The E-PL 10×/20 Br. eyepieces are intended for use with eyepiece reticles (see overview under 1. 6). The slight image shift caused by the additional path through the glass is taken into account on the diopter scale by the fact that the zero point position is indicated not by the white dot (2-3/W) but by the red dot (2-3/R). The eyepiece reticles (2-3/1) have been adhered to screw-in mounts (2-3/2) to allow easy replacement. Fig. 2-3 Insertion of eyepiece reticle · To replace a reticle, unscrew the screw-on mount containing the eyepiece reticle and replace it with the required one. If eyepiece reticles are inserted into the unscrewed mount by the customer, attention must be paid to the labelling being visible the right way up after insertion. 2-4 B 40-015 e 06/99 Axiolab A Carl Zeiss 2. 3. 2 Compensation of Ametropia when Eyepiece Reticles are used The correct use of an eyepiece reticle requires two focusing eyepieces, e. g. foc. , to enable compensation of ametropia. · Use the eyelens of the focusing eyepiece to focus on the line figure of the eyepiece reticle; focus on the edge of the field of view if no eyepiece reticle is used. · Focus on the microscope image of a specimen via the focusing drive by looking through the eyepiece with reticle. · When the image and the eyepiece reticle are in focus in the above eyepiece, focus the image for the second eye via the focusing eyelens of the second eyepiece. The position of the focusing drive on the stand must not be changed. 2. 4 Setting of Interpupillary Distance · The eyepiece distance is matched to the individual interpupillary distance by swinging the eyepiece tubes symmetrically towards one another. The eyepiece distance can be set in the range between 55 and 75 mm, and you should try to remember the individual value once set. B 40-015 e 06/99 2-5 Carl Zeiss Axiolab A 2. 5 Attachment of Reflected-Light Halogen Illuminator · Attach dovetail guide (2-4/3) of 6 V, 30 W halogen lamp (2-4/4) to the microscope stand and tighten hexagonal screw (2-4/2) using the SW 3 screwdriver (2-4/1). The halogen lamp must be mounted to the stand in a vertical position, also see Fig. For visual control, remove lamp cover (2-4/4) by slightly turning it to the left. · Connect cable (2-4/5) to the two sockets on the instrument rear. 2. 6 Retrofit the Transmitted-Light Halogen Illumination Fig. 2-4 Attachment of reflected-light halogen illuminator Transmitted-light observation with the Axiolab A is possible with the configuration described in section 1. 3. The pure reflected-light versions of the Axiolab A can also be upgraded for transmittedlight observation by installing the "Retrofit package for transmitted light" (1-4/7). The transmitted-light illumination equipment can be subsequently mounted to the Axiolab A only by Carl Zeiss service personnel. The transmitted-light illumination package consists of: - mechanical stage 75 × 35 R/A with ceramic surface and short drive, - specimen holder with spring clip R, - transmitted-light illuminator, containing halogen lamp with mount and luminous-field diaphragm with iris, - ABBE condenser 0. 9/1. 25 or ABBE condenser 0. 9/1. 25 with 5-position turret disk 2-6 B 40-015 e 06/99 Axiolab A Carl Zeiss 2. 6. 1 Switch on the Transmitted-Light Halogen Illuminator Normally, the position of the A/D illumination converter (1-5/13 or 2-7/4) is of no importance with the Axiolab A. Only if the transmitted-light illumination is a standard feature or has been subsequently installed, the A/D converter will have the following functions: · the power supply for the reflected-light halogen illuminator is switched on in the upper, pressed position of the converter. · in the lower, pressed position of the converter, the power supply for the transmitted-light illuminator is switched on. To change to transmitted-light operation, press the A/D converter (1-5/13) at the rear of the Axiolab A into the lower position. The functions of the on/off switch (1-5/28) and the "illuminance" control (1-5/27) are retained, regardless of the position of the AD converter A/D (1-5/13) (see section 1. 5). 2. 7 Equipment of Filter Slider · Equip filter slider (2-5/3) with the filters (2-5/2) required for the relevant microscopy technique, e. g. with - neutral-density filter N 0. 06 (6% transmission) - neutral-density filter N 0. 25 (25% transmission) - conversion filter 3200. . . 5500 K for use of daylight color film - narrow-band interference filter, e. g. for contrast enhancement Insert equipped filter slider in the compartment until it click-stops in the relevant position in the reflected-light illuminator (2-5/1). Fig. 2-5 Equipment of filter slider The filter diameter is 32 mm. B 40-015 e 06/99 2-7 Carl Zeiss Axiolab A 2. 8 Set the Luminous-Field Diaphragm · The pushrod (2-6/1) for the luminous-field diaphragm has two positions: - pushed in: the full field of view is visible without restriction. [. . . ] 4-1 Changing the fuses · Insert fuse holder again until the springs at the side can be heard to engage. · Connect the instrument to the line. 4-4 B 40-015 e 06/99 Axiolab A Carl Zeiss (2) Changing the 6 V, 30 W Reflected-Light Halogen Lamp The following procedure is required to change the lamp of the 6 V, 30 W reflected-light halogen illuminator: · Switch off the lamp supply; if required, allow the illuminator to cool down for approx. · Disconnect the line cable from the line. · Remove the connection cable of the reflectedlight illuminator from the socket at the rear of the instrument. [. . . ]