User manual ZEISS AXIOVERT 200M

[. . . ] Operating Manual Axiovert 200 / Axiovert 200 M Inverted Microscopes Carl Zeiss INTRODUCTION Copyright Axiovert 200 Knowledge of this manual is required for the operation of the instrument. Would you please therefore make yourself familiar with the contents of this manual and pay special attention to hints concerning the safe operation of the instrument. The specifications are subject to change; the manual is not covered by an update service. © Unless expressly authorized, forwarding and duplication of this document, and the utilization and communication of its contents are not permitted. [. . . ] The objective table below specifies the hole size for the most common objectives that must be cut out for the corresponding objective. · Put cover mat onto nosepiece. Press down the cover mat only as far as is possible without noticeably slowing down the motion of the nosepiece. · Next, put a silicone ring (2-38/3) over every objective. There are two types of silicone rings available: Size 1 ­ small, Size 2 ­ large. The objective table also specifies the size of the silicone ring to be used for the most common objectives. On objectives with correction ring, the silicone ring must be above the correction ring. On all other objectives, slide the silicone ring up to about 3 mm before the beginning of the thicker part of the objective to ensure correct function of the objective's specimen protection mechanism. · Then, slip the cut drain hose over the two drain connectors (2-38/6). Put the other end of the hose into the collecting tank. The liquid can only drain if the collecting tank provides venting. by a hole in the lid or by screwing the lid only loosely. 2-28 B 40-080 e 03/01 Axiovert 200 START-UP Attachment of Aqua Stop Carl Zeiss · You can fix the drain hose using the provided clips. Fix the hose in such a way, that the drain gutter is not bent in focusing. · Attach the microscope stage again. After any accident with liquids, you are strongly advised to remove the microscope stage and dab off any liquid drops using a lintless cloth. Particularly, clean the front lenses of the objectives to be able to fully utilize the full performance of the objective. Objective type Magnification / num. aperture Contrasting Hole size Silicone ring size Cat. No. A-Plan A-Plan A-Plan A-Plan A-Plan A-Plan LD A-Plan LD A-Plan LD A-Plan LD A-Plan LD A-Plan LD A-Plan LD Achroplan Achroplan Achroplan LD Achroplan LD Achroplan LD Achroplan LD Achroplan LD Achroplan Fluar Fluar Fluar Fluar 5x/0. 12 10x/0. 20 20x 40x 100x 20x/0. 30 20x/0. 30 32x/0. 40 32x/0. 35 40x/0. 50 40x/0. 50 ph0 ph1 var1 ph2 Ph2 ph3 ph1 ph1 var1 ph1 ph1 var1 ph2 ph2 var2 2 2 2 3 3 2 2 2 2 2 2 1 1 1 1 1 1 1 1 1 1 1 000000-1018-589 000000-1020-863 441041-0000-000 441051-0000-000 441080-0000-000 000000-1006-591 000000-1006-592 000000-1006-593 000000-1006-594 000000-1006-595 000000-1006-596 4x/0. 10 10x/0. 25 20x/0. 40 20x/0. 40 40x/0. 60 40x/0. 60 63x/0. 75 ph1 ph2 ph2 ph2 2 2 3 3 4 4 4 1 1 1 1 2 2 2 440020-0000-000 440031-0000-000 440844-0000-000 440845-0000-000 440864-0000-000 440865-0000-000 440861-0000-000 10x 20x 40x/1. 3 Iris 3 3 4 1 1 1 440135-0000-000 440145-0000-000 440257-0000-000 B 40-080 e 03/01 2-29 Carl Zeiss START-UP Attachment of Aqua Stop Axiovert 200 Objective type Magnification / num. aperture Contrasting Hole size Silicone ring size Cat. No. Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Neofluar Plan-Apochromat Plan-Apochromat Plan-Apochromat 5x/0. 15 5x/0. 15 10x/0. 30 10x/0. 30 16x/0. 50 Imm 16x/0. 50 Imm 20x/0. 50 20x/0. 50 40x/0. 75 40x/0. 75 40x/1. 30 Oil 40x/1. 30 Oil 63x/0. 95 Korr. 63x/1. 25 Oil Iris 100x/1. 30 Oil 100x/1. 30 Oil ph1 ph1 ph1 ph2 ph2 ph3 ph3 2 2 2 2 3 3 2 2 2 2 3 3 3 4 2 2 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 440320-0000-000 440321-0000-000 440330-0000-000 440331-0000-000 440530-0000-000 440531-0000-000 000000-1004-072 000000-1004-989 440350-9902-000 440351-9902-000 000000-1022-818 000000-1022-819 440364-0000-000 440466-0000-000 000000-1018-595 000000-1031-171 63x/1. 40 Oil 10x0. 45 4 3 1 1 440760-0000-000 440639-0000-000 2-30 B 40-080 e 03/01 Axiovert 200 OPERATION Contents Carl Zeiss OPERATION Contents 3 3. 1 3. 1. 1 3. 1. 2 3. 2 3. 2. 1 3. 2. 2 3. 3 3. 3. 1 3. 3. 2 3. 3. 3 3. 3. 4 3. 3. 5 3. 4 3. 4. 1 3. 4. 2 3. 4. 3 OPERATION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3 Axiovert 200 (manual) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4 Operation and function controls on the Axiovert 200 (manual) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4 Switching on and basic settings on the Axiovert 200 (manual) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-14 Axiovert 200 M (motorized) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16 Operation and function controls on the Axiovert 200 (motorized) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16 Switching on and basic settings on the Axiovert 200 M (motorized) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-23 Illumination and contrasting techniques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-28 Setting of transmitted-light brightfield for KÖHLER illumination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-28 Setting of transmitted-light phase contrast . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . [. . . ] 3-43 installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5 iris stop slider . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13 K key HAL on/off . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6 KÖHLER . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 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